Abstract: The pharmacokinetic interaction between bencycloquidium bromide nasal spray(BCQB)and budesonide nasal spray(BUD)in human was investigated. 12 male volunteers were randomly divided into two groups according to an open, randomized, two-period, two-way crossover design. In period 1, Group I took single dose BCQB 180 μg, multiple doses BCQB(180 μg, four times daily for 6 days)and combined dose(BCQB 180 μg and BUD 256 μg); Group II took single dose BUD 256 μg, multiple doses BUD(256 μg, once daily for 7 days)and combined dose(BCQB 180 μg and BUD 256 μg), successively. After a wash-out time of 7 days, the two groups exchanged their administration processes in period 2. Plasma concentration of bencycloquidium bromide and budesonide were measured by LC-MS/MS, respectively. The pharmacokinetic parameters, c_max, AUC and t_max, were calculated and statistically analyzed. Results showed that there was no obvious pharmacokinetic interaction between BUD and BCQB when they were administered jointly to healthy volunteers.