考布他汀A-4衍生物CPU-XT-006对人脐静脉内皮细胞增殖、凋亡及VEGF 、bFGF表达的影响

Effect of combretastatin A-4 derivative CPU-XT-006 on proliferation, apoptosis and expression of VEGF and bFGF of HUVEC in vitro

摘要: 化合物CPU-XT-006是微管蛋白聚合抑制剂、血管阻断剂考布他汀A-4(CA-4)的一种衍生物。通过MTT法分别检测CPU-XT-006对血清、血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)分别诱导的人脐静脉内皮细胞(HUVEC)增殖的影响；采用流式细胞仪检测CPU-XT-006对HUVEC凋亡的影响；采用Western blot法检测CPU-XT-006对HUVEC内VEGF、bFGF细胞因子表达的影响。结果显示，CPU-XT-006可抑制HUVEC的增殖，诱导HUVEC凋亡，并降低其细胞内VEGF、bFGF细胞因子的表达。

Abstract: Compound CPU-XT-006 is a derivative of combretastatin A-4(CA-4), one kind of microtubule protein polymerization inhibitors and vascular blocking agents. MTT assay was used to detect the effect of CPU-XT-006 on the multiplication of HUVEC induced by newborn calf serum, vascular endothelial gronth factor(VEGF)or basic fibroblast growth tactor(bFGF)respectively. Flow cytometry was used to detect the HUVEC apoptosis caused by CPU-XT-006. The expression of VEGF and bFGF protein was detected by Western blot. The results showed that the compound CPU-XT-006 could inhibit HUVEC proliferation, induce HUVEC apoptosis and reduce expression of VEGF and bFGF protein.