Abstract: This study was aimed to establish the ischemia-reperfusion(I/R)myocardial cells injury model using microfluidic technology in vitro. This model was fast and efficient to be used for drug screening especially in the field of cardiovascular drug. In the present study, we firstly made a fan-shaped microfluidic chip using soft lithography technology and molding technology, then the myocardial cells were cultured in this chips. Then the cells were exposed to ischemic tyrode′s reagents to mimic the I/R injury followed by the cell viability analysis. The morphology and the content of damage index were analyzed by Wright-Giemsa dye and ELISA kit, respectively. Finally, puerarin was used to evaluate the efficiency of this model since Compared with the control group, the cell viability of myocardial cells cultured in this chip was up to 98% in the chip. Also, there was not significant diffe-rence of myocyte apoptosis and damage index′ content between cells cultured in normal condition and that cultured in chip. Furthermore, puerarin treatment ameliorated the myocyte apoptosis, and the content of damage index induced by the I/R injury. Therefore, we successfully established the injury model based on microfluidic technology in vitro, providing novel idea and method for drug screening in the field of cardiovascular repair by microfluidic technology.