Abstract: To investigate the effects of colchicine on the cholestasis in rats induced by bile duct ligation(BDL), colchicine(0. 12 and 0. 24 mg/kg)was administered intragastrically for 7 consecutive days after ligation of common bile duct. The effect of colchicines on the liver injury was evaluated by serum biochemistry(ALT, AST, ALP, γ-GTP, TBIL, DBIL, ALB and TBA), liver myeloperoxidase(MPO)activity as well as histological examination. Both doses of colchicine significantly decreased serum ALP, γ-GTP and TBA levels, inhibited liver MPO activity and alleviate liver histological changes, such as inflammatory cell infiltration and interlobular bile duct hyperplasia. Data suggested that lower dose of colchicines(≤0. 24 mg/kg)exerted protection of bile duct injury induced by BDL in rats involving primary mechanism on inhibiting activation and infiltration of inflammatory cells.