Abstract: To screen ginsenosides with inhibitory activities on the activation of NLRP3 inflammasome, 17 ginsenosides(PPT, PPD, Rb1, Rb2, Rb3, Rc, Rd, Re, Rg1, Rg2, Gg₃, Rh1, Rh2, Ro, Rh2, Ro, cK, F1, F2)were used to treat mouse peritoneal macrophages(PMs)induced with NLRP3 inflammasome inducers(ATP, nigericin, or silica). In the present study, the higher anti-inflammatory activity of ginsenoside monomers were confirmed by analysis of IL-1β secretion in PMs. In the group of ATP induction, IL-1β secretion decreased after ginsenoside treatments, the high inhibitory effect was found at treatments of PPT, PPD, and F1(P< 0. 001). At the group of the nigericin, Rg3 also possessed higher inhibitory effect on IL-1β secretion except PPT, PPD, and F1(P< 0. 001). Furthermore, compared with groups of the ATP and nigericin, IL-1β secretion at the group of silica decreased after treatments of various ginsenoside monomers, including PPT, PPD, Rd, Rg3, Rb3, Rb2, F2, Re, F1, and Rh2(P< 0. 001). The present study indicates that PPT, PPD, and F1 can efficiently inhibit NLRP3 inflammasome activation induced by different inducers.