Abstract: DNA nanocages are nanoscale three-dimensional polyhedral structures formed by self-assembled oligonucleotide strands by base pairing. DNA nanocages, especially the tetrahedron DNA nanocages, were proved to be potential nanocarriers for drug delivery, due to their good stablility, high compatibility, intracellular ablility and hollow cavity for modification and drug loading. . In this paper, we reviewed the structural characteristics, synthetic methods and targeted modification of DNA nanocages, as well as their development and application in drug carriers, biological imaging, in vitro diagnosis and materials science.