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Anisopus mannii 叶的化学成分和抑制黑色素生成活性研究

叶峰, 吕青林, 祝婉芳, 冯锋, 张杰

叶峰, 吕青林, 祝婉芳, 冯锋, 张杰. Anisopus mannii 叶的化学成分和抑制黑色素生成活性研究[J]. 中国药科大学学报, 2018, 49(6): 676-681. DOI: 10.11665/j.issn.1000-5048.20180606
引用本文: 叶峰, 吕青林, 祝婉芳, 冯锋, 张杰. Anisopus mannii 叶的化学成分和抑制黑色素生成活性研究[J]. 中国药科大学学报, 2018, 49(6): 676-681. DOI: 10.11665/j.issn.1000-5048.20180606
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YE Feng, LYU Qinglin, ZHU Wanfang, FENG Feng, ZHANG Jie. Chemical constituents from the leaves of Anisopus mannii and the melanogenesis inhibitory activities[J]. Journal of China Pharmaceutical University, 2018, 49(6): 676-681. DOI: 10.11665/j.issn.1000-5048.20180606
Citation: YE Feng, LYU Qinglin, ZHU Wanfang, FENG Feng, ZHANG Jie. Chemical constituents from the leaves of Anisopus mannii and the melanogenesis inhibitory activities[J]. Journal of China Pharmaceutical University, 2018, 49(6): 676-681. DOI: 10.11665/j.issn.1000-5048.20180606
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Anisopus mannii 叶的化学成分和抑制黑色素生成活性研究

  • 1.

    中国药科大学中药学院

  • 2.

    江苏食品药品职业技术学院

基金项目: 国家自然科学基金资助项目(No.81703383)；江苏省自然科学基金资助项目(No.BK20170742)；国家基础科学人才培养基金资助项目(No.J1310032)

Chemical constituents from the leaves of Anisopus mannii and the melanogenesis inhibitory activities

摘要

    摘要
  • 摘要: 采用硅胶、ODS和大孔树脂等柱色谱分离方法对Anisopus mannii的叶进行分离纯化，通过NMR、HR-ESI-MS等波谱技术鉴定了7个化合物分别为:3β-acetoxylup-20(29)-ene( 1 )，1-acetoxy-2-isopropyl-1-tridecene( 2 )，rutin( 3 )，3，6′-diferuloylsucrose( 4 )，3-O-β-D-glucopyranosyl-3-β-hydroxyolean-12-en-28-oic acid 28-O-［α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranosyl］ ester( 5 )，conduritol A( 6 )，hoyacarnoside I( 7 )。同时采用B16细胞为黑色素抑制的筛选模型，筛选了所有化合物的黑色素抑制活性和细胞毒性。结果显示:所有化合物均具有不同程度的抑制黑色素生成的活性，其中化合物 5 显示出最优活性［30 μmol/L给药浓度，黑色素含量:(27.4±3.5)%，细胞存活率:(54.9±5.6)%］，可以作为美白剂进一步开发。
    Abstract: The phytochemical studies on the leaves of Anisopus mannii led to the isolation of seven compounds by silica gel, ODS, DIAION HP-20, Sephadex LH-20 colunmn chromatographer, their structures were elucidated on the basis of the spectroscopic analyses(NMR, HRMS)and the comparisons with the literatures as 3β-acetoxylup-20(29)-ene( 1 ), 1-acetoxy-2-isopropyl-1-tridecene( 2 ), rutin( 3 ), 3, 6′-diferuloylsucrose( 4 ), 3-O-β-D-glucopyranosyl-3-β-hydroxyolean-12-en-28-oic acid 28-O-［α-L-rhamnopyra- nosyl-(1→2)-β-D-glucopyranosyl］ ester( 5 ), conduritol A( 6 ), hoyacarnoside I( 7 ). Meanwhile, the isolated compounds were evaluated for their inhibitory activities against melanogensis in B16 melanoma cells, as the results, all compounds exhibited melanogenesis-inhibitory activity and compound 5 showed a strongest activity(Melanin content: (27. 4±3. 5)%, Cell Viability: (54. 9±5. 6)% with a concentration of 30 μmol/L)which could be further developed.
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  • 刊出日期:  2018-12-24

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