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富硒黄芪提取物对链脲霉素诱导的糖尿病大鼠胰岛素抵抗的影响

李永荣, 程涛, 王永胜, 李钦, 高博, 贺云, 白宇

李永荣, 程涛, 王永胜, 李钦, 高博, 贺云, 白宇. 富硒黄芪提取物对链脲霉素诱导的糖尿病大鼠胰岛素抵抗的影响[J]. 中国药科大学学报, 2018, 49(6): 739-745. DOI: 10.11665/j.issn.1000-5048.20180616
引用本文: 李永荣, 程涛, 王永胜, 李钦, 高博, 贺云, 白宇. 富硒黄芪提取物对链脲霉素诱导的糖尿病大鼠胰岛素抵抗的影响[J]. 中国药科大学学报, 2018, 49(6): 739-745. DOI: 10.11665/j.issn.1000-5048.20180616
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LI Yongrong, CHENG Tao, WANG Yongsheng, LI Qin, GAO Bo, HE Yun, BAI Yu. Effect of extract of selenium-enriched Astragalus membranaceus on insulin resistance in streptozotocin-induced diabetic rats[J]. Journal of China Pharmaceutical University, 2018, 49(6): 739-745. DOI: 10.11665/j.issn.1000-5048.20180616
Citation: LI Yongrong, CHENG Tao, WANG Yongsheng, LI Qin, GAO Bo, HE Yun, BAI Yu. Effect of extract of selenium-enriched Astragalus membranaceus on insulin resistance in streptozotocin-induced diabetic rats[J]. Journal of China Pharmaceutical University, 2018, 49(6): 739-745. DOI: 10.11665/j.issn.1000-5048.20180616
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富硒黄芪提取物对链脲霉素诱导的糖尿病大鼠胰岛素抵抗的影响

  • 1.

    甘肃中医药大学附属医院

  • 2.

    甘肃省中医药研究院

  • 3.

    甘肃省中医院

  • 4.

    甘肃卫生职业学院

  • 5.

    甘肃中医药大学

基金项目: 甘肃省中医药管理局资助项目(No.GZK-2016-20)

Effect of extract of selenium-enriched Astragalus membranaceus on insulin resistance in streptozotocin-induced diabetic rats

摘要

    摘要
  • 摘要: 为探索富硒黄芪提取物(extract of selenium-enriched Astragalus membranaceus，ESAM)对链脲霉素(STZ)诱导的糖尿病大鼠胰岛素抵抗(insulin resistance，IR)的作用。用STZ制造2型糖尿病大鼠模型(type 2 diabetes mellitus，T2DM)；ESAM和吡格列酮干预T2DM模型大鼠；观察各组情况，并于造模前、造模后记录体重和空腹血糖(FBG)；第4周末，收集各组大鼠血液及肝脏组织；生化检测仪检测血液中总胆固醇(CHOL)、三酰甘油(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)；ELISA检测血液中空腹胰岛素(FINS)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、C-反应蛋白(CRP)、白细胞介素1β(IL-1β)的含量变化；Western blot检测肝脏组织中胰岛素受体底物蛋白1(IRS1)、磷酸化IRS1(p-IRS1)、核因子κB抑制子激酶β(IKKβ)、磷酸化IKKβ(p-IKKβ)、c-Jun氨基末端激酶(JNK)、磷酸化JNK(p-JNK)蛋白变化。实验结果表明，与空白组相比，模型组大鼠体重显著下降，血液中FBG、CHOL、TG、LDL、TNF-α、IL-6、CRP、IL-1β以及肝脏中p-IRS1、p-IKKβ、p-JNK显著上升；药物干预后，吡格列酮组、ESAM可逆转模型大鼠体重及上述细胞因子和蛋白的变化。综上可知ESAM可通过降低T2DM大鼠炎症因子的表达，抑制游离脂肪酸(FFA)的升高，降低周围组织中IKKβ和JNK磷酸化活化，进而激活胰岛素通路，改善T2DM的IR作用。
    Abstract: To explore the effects of extract of selenium-enriched Astragalus membranaceus(ESAM)on insulin resistance(IR)in streptozotocin(STZ)diabetic rats. In this study, type 2 diabetes mellitus(T2DM)was established; ESAM and pioglitazone were used to intervene T2DM model rats; the general condition of rats in each group was observed, and body weight and fasting blood glucose(FBG)were recorded before modeling and after modeling. At the end of the fourth week, the blood and liver tissues of each group were collected. The total cholesterol(CHOL), triglyceride(TG), high-density lipoprotein(HDL), low-density lipoprotein(LDL)in blood were detected by biochemical detector; ELISA was used to detected content changes of blood fasting insulin(FINS), tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), C-reactive protein(CRP), interleukin-1β(IL-1β); Western blotting was used for the detection of insulin receptor substrate protein 1(IRS1), phosphorylated IRS1(p-IRS1), nuclear factor κB inhibitor kinase β(IKKβ), phosphorylated IKKβ(p-IKKβ), c -Jun N-terminal kinase(JNK), phosphorylated JNK(p-JNK)protein changes. Results showed that compared with the blank group, the body weight of the model group was significantly decreased. FBG, CHOL, TG, LDL, TNF-α, IL-6, CRP, IL-1β in the blood and p-IRS1, p-IKKβ, p-JNK in the liver was increased significantly; after intervention, pioglitazone, ESAM can reverse the body weight of the model rats and the changes of the above cytokines and proteins. In conclusion, ESAM can reduce the expression of inflammatory cytokines in T2DM rats, inhibit the increase of free fatty acids(FFA), decrease the activation of IKKβ and JNK phosphorylation in surrounding tissues, activate insulin pathway and improve the IR effect of T2DM.
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  • 刊出日期:  2018-12-24

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