绞股蓝总苷颗粒改善高脂血症C57BL/6J小鼠脂代谢的研究
Gypenoside granules improved lipid metabolism in C57BL/6J mice with hyperlipidemia
-
摘要: 为评价绞股蓝总苷颗粒以及其与他汀联用的降血脂作用，采用高脂饲料诱导构建高脂血症C57BL/6J小鼠模型。随机分为空白组、模型组、阳性药组(Lip，10 mg/kg)、绞股蓝总苷颗粒低、中、高剂量组(L、M、H，90，120，180 mg/kg)，联合组(Lip+H，180 mg/kg+10 mg/kg)。连续给药4周后检测血脂成分、血清ALT、AST及载脂蛋白B(Apo B)含量，H&E染色观察小鼠肝脏病理变化，利用RT-PCR法和Western blot法测定三磷酸腺苷结合盒转运体A1(ABCA1)、肝X受体(LXRα)、胆固醇7α-羟化酶(CYP7A1)和B族Ι型清道夫受体(SR-BΙ)等肝脏胆固醇代谢关键因子蛋白、基因表达水平。结果显示绞股蓝总苷颗粒能显著降低小鼠体重、腹部脂肪质量及血清总胆固醇(TC)、AST水平，并修复受损小鼠肝细胞；联合组能显著降低小鼠TC水平，效果优于绞股蓝总苷颗粒高剂量组和阳性药单用组。此外，绞股蓝总苷颗粒能显著升高ABCA1的蛋白水平，同时上调ABCA1、CYP7A1和SR-BΙ的基因表达。实验结果表明，绞股蓝总苷颗粒能够显著降低血脂，且与阿托伐他汀联用后降脂效果提高，并可降低血清转氨酶，初步探索其降脂机制可能是通过参与胆固醇逆转运调节TC含量。Abstract: To investigate the hypolipidemic effects of gypenosides granules and its combination with lipitor, a model of hyperlipidaemia C57BL/6J mice was established by high-fat diet feeding for 4 weeks. The mice were randomly divided into blank group, model group, lipitor group(10 mg/kg of lipitor), low dose group(90 mg/kg of gypenosides granules), medium dose group(120 mg/kg of gypenosides granules), high dose group(180 mg/kg of gypenosides granules)and the combination group(180 mg/kg of gypenosides granules and 10 mg/kg of lipitor). After 4 weeks of continuous administration, the contents of serum lipid indexes, serum ALT, AST and apolipoprotein B(ApoB)were measured. The liver tissues of mice were observed by H&E staining. The expression levels of key factors involved in hepatic cholesterol metabolism were observed by RT-PCR and Western blot methods, such as adenosine triphosphate combined box transporter A1(ABCA1), liver X receptor(LXRα), cholesterol 7 alpha hydroxylase(CYP7A1)and type BΙ scavenger receptor(SR-BΙ). The results revealed that gypenosides granules significantly decreased the mice body weight, total abdominal fat area and the level of serum total cholesterol(TC). The combination group showed a more significant reduction in TC level than the other administration groups. Moreover, gypenosides granules treatment remarkably increased the protein expression of ABCA1 and up-regulated the mRNA expression of ABCA1, CYP7A1 and SR-BI. The above results suggest that gypenosides granules can significantly reduce blood lipid contents, and the combination therapy with lipitor show better the lipid-lowering effect. Meanwhile, gypenosides granules can decrease the level of serum transaminase. Preliminary exploration suggests the lipid-lowering mechanism of gypenosides granules may be involved in cholesterol reversal to regulate the level of TC.
-
-
[1] Catapano AL,Graham I,De Backer G, et al.2016 ESC/EAS guidelines for the management of dyslipidaemias[J].Eur Heart J,2016,37(39):2999-3058. [2] Balaz M,Becker AS,Balazova L, et al.Inhibition of mevalonate pathway prevents adipocyte Browning in mice and men by affecting protein prenylation[J].Cell Metab,2019,29(4):901-916. [3] Klose G,Beil FU,Dieplinger H, et al.New AHA and ACC guidelines on the treatment of blood cholesterol to reduce atherosclerotic cardiovascular risk[J].Wien Klin Wochenschr,2014,126(5/6):169-175. [4] Vasudevan AR,Hamirani YS,Jones PH.Safety of statins:effects on muscle and the liver[J].Cleve Clin J Med,2005,72(11):990-993,996-1001. [5] Li Y,Wang X,Shen Z.Traditional Chinese medicine for lipid metabolism disorders [J].Am J Transl Res, 2017,9(5):2038-2049. [6] Hasani-Ranjbar S,Nayebi N,Moradi L, et al.The efficacy and safety of herbal medicines used in the treatment of hyperlipidemia; a systematic review[J].Curr Pharm Des,2010,16(26):2935-2947. [7] Razmovski-Naumovski V,Huang THW,Tran VH, et al.Chemistry and pharmacology of Gynostemma pentaphyllum[J].Phytochem Rev,2005,4(2/3):197-219. [8] Peng L,Li YJ,C J, et al.Study on the total Saponins and polysaccharides content in Gynostemma pentaphyllum from different producing areas and different varieties [J].Aisa-Pacific Tradi Med(亚太传统医药),2015,22(11):21-24. [9] Wong WY,Lee MM,Chan BD, et al.Gynostemma pentaphyllum saponins attenuate inflammation in vitro and in vivo by inhibition of NF-κB and STAT3 signaling[J].Oncotarget,2017,8(50):87401-87414. [10] Gauhar R,Hwang SL,Jeong SS, et al.Heat-processed Gynostemma pentaphyllum extract improves obesity in ob/ob mice by activating AMP-activated protein kinase[J].Biotechnol Lett,2012,34(9):1607-1616. [11] Itariu BK,Stulnig TM.Obesity,insulin resistance,and inflammaging[M]//Inflammation,Advancing Age and Nutrition,2014:157-164. [12] Wang XT,Zhang M,Guo HF, et al.Effect and mechanism of atorvastatin on blood glucose of mild diabetic rats[J].J China Pharm Univ(中国药科大学学报),2012,43(5):453-459. [13] Yeo J,Kang YJ,Jeon SM, et al.Potential hypoglycemic effect of an ethanol extract of Gynostemma pentaphyllum in C57BL/KsJ-db/Db mice[J].J Med Food,2008,11(4):709-716. [14] Liu J,Li YF,Yang PY, et al.Gypenosides reduced the risk of overweight and insulin resistance in C57BL/6J mice through modulating adipose thermogenesis and gut Microbiota[J].J Agric Food Chem,2017,65(42):9237-9246. [15] Lu YL,Du YM,Qin L, et al.Gypenosides altered hepatic bile acids homeostasis in mice treated with high fat diet[J].Evid Based Complement Alternat Med,2018,2018:8098059. [16] Escolà-Gil JC,Rotllan N,Julve J, et al.In vivo macrophage-specific RCT and antioxidant and antiinflammatory HDL activity measurements:new tools for predicting HDL atheroprotection[J].Atherosclerosis,2009,206(2):321-327. [17] Wang SH,Smith JD.ABCA1 and nascent HDL biogenesis[J].Biofactors,2014,40(6):547-554. [18] Zou B,Li CM,Chen JY, et al.High molecular weight persimmon tannin is a potent hypolipidemic in high-cholesterol diet fed rats[J].Food Res Int,2012,48(2):970-977. [19] Dávalos A,Fernández-Hernando C.From evolution to revolution:miRNAs as pharmacological targets for modulating cholesterol efflux and reverse cholesterol transport[J].Pharmacol Res,2013,75:60-72. [20] Jiao R,Zhang ZS,Yu HJ, et al.Hypocholesterolemic activity of grape seed proanthocyanidin is mediated by enhancement of bile acid excretion and up-regulation of CYP7A1[J].J Nutr Biochem,2010,21(11):1134-1139. [21] Tu Z,Moss-Pierce T,Ford P, et al.Rosemary(Rosmarinus officinalis L.)extract regulates glucose and lipid metabolism by activating AMPK and PPAR pathways in HepG2 cells[J].J Agric Food Chem,2013,61(11):2803-2810. [22] Fu ZD,Cui JY,Klaassen CD.Atorvastatin induces bile acid-synthetic enzyme Cyp7a1 by suppressing FXR signaling in both liver and intestine in mice[J].J Lipid Res,2014,55(12):2576-2586. -
期刊类型引用(10)
1. 王康，肖佳佳，黄琦，李维旭. 李维旭教授辨体质结合茶疗论治高脂血症经验. 实用中西医结合临床. 2025(03): 84-86+120 . 
百度学术
2. 李孟莲，章莹玺，佘振南，杨长鑫，冯宝文，施和敏，严冬，周珊珊，罗洪，杨鑫嵎，朱体贞，陈彤. 绞股蓝总苷药理活性及其作用机制研究进展. 云南化工. 2025(04): 1-6 . 百度学术
3. 白宇，陈晓虎，韩捷，范群丽，陈理，李文友，赵静，朱琼，蔚建斌，王石. 基于数据挖掘探讨唐蜀华教授治疗冠心病的用药规律. 中西医结合心脑血管病杂志. 2024(05): 857-863 . 百度学术
4. 曾迪，周昌园，龙健，胡雪黎，涂星，文德鉴，胡泽华，杨宝. 基于脂质组学的绞股蓝总苷调血脂作用研究. 中草药. 2023(04): 1149-1156 . 百度学术
5. 王振兴，杨金梅，张志斌，朱晓湉，向萍，孙健，何霞红. 绞股蓝的化学成分及其生物活性研究进展. 南方农业学报. 2023(06): 1741-1752 . 百度学术
6. 代谢综合征病证结合诊疗指南. 世界中医药. 2023(22): 3157-3166 . 百度学术
7. 崔馨月，贾连群，杨关林，吕美君，王群. 绞股蓝总苷对骨质疏松合并动脉粥样硬化小鼠的防治作用. 中华中医药学刊. 2022(09): 193-196+284-285 . 百度学术
8. 王云杉，王杰，王潇楠，蒋翠花，郑显，张健，殷志琦. 绞股蓝总皂苷治疗高胆固醇血症及其对肝损伤的保护作用. 中国药科大学学报. 2021(01): 84-91 . 本站查看
9. 娄云云，牟龙，黄亚萍，张健，殷志琦，潘珂. 绞股蓝中1个新的葫芦烷型皂苷类化合物. 中草药. 2021(07): 1872-1876 . 百度学术
10. 糖尿病合并高脂血症病证结合诊疗指南. 世界中医药. 2021(16): 2377-2385 . 百度学术
其他类型引用(14)
计量
- 文章访问数: 586
- HTML全文浏览量: 3
- PDF下载量: 600
- 被引次数: 24
下载:
