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王丽新, 姜修博, 郭巧珍, 王籽橙, 王勃, 王玉霞, 瞿文生, 段小涛. TM9SF2促进三阴性乳腺癌MDA-MB-231细胞的增殖与转移[J]. 中国药科大学学报, 2021, 52(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20210514
引用本文: 王丽新, 姜修博, 郭巧珍, 王籽橙, 王勃, 王玉霞, 瞿文生, 段小涛. TM9SF2促进三阴性乳腺癌MDA-MB-231细胞的增殖与转移[J]. 中国药科大学学报, 2021, 52(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20210514
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WANG Lixin, JIANG Xiubo, GUO Qiaozhen, WANG Zicheng, WANG Bo, WANG Yuxia, QU Wensheng, DUAN Xiaotao. TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231[J]. Journal of China Pharmaceutical University, 2021, 52(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20210514
Citation: WANG Lixin, JIANG Xiubo, GUO Qiaozhen, WANG Zicheng, WANG Bo, WANG Yuxia, QU Wensheng, DUAN Xiaotao. TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231[J]. Journal of China Pharmaceutical University, 2021, 52(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20210514
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TM9SF2促进三阴性乳腺癌MDA-MB-231细胞的增殖与转移

TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231

    摘要
  • 摘要: 探究9次跨膜超家族蛋白2（transmembrane 9 superfamily protein member 2，TM9SF2）对于三阴性乳腺癌MDA-MB-231细胞增殖和转移的影响及其分子机制。采用Western blot实验检测三阴性乳腺癌细胞株MDA-MB-231和非致瘤的乳腺上皮细胞株MCF-10A中TM9SF2蛋白表达的情况；对高表达TM9SF2的三阴性细胞株MDA-MB-231进行基因沉默；采用MTS法检测细胞增殖活性，采用Transwell实验和划痕实验检测细胞的转移能力；采用Western blot实验检测细胞内增殖相关蛋白（PI3K、AKT、SRC和ERK）和转移相关蛋白（Snail、Slug和N-cadherin）的表达情况。Western blot实验证明，MDA-MB-231中TM9SF2蛋白的表达量高于MCF-10A细胞。与对照组相比，siRNA-TM9SF2转染组TM9SF2蛋白表达下调，细胞增殖活性降低，细胞转移能力减弱，PI3K、Snail、Slug和N-cadherin表达水平均降低，AKT蛋白磷酸化激活降低。研究结果表明，TM9SF2基因能促进三阴型乳腺癌MDA-MB-231细胞的增殖和转移。

     

    Abstract: The aim of this study was to investigate the effect of transmembrane 9 superfamily protein member 2 (TM9SF2) in proliferation and migration of triple negative breast cancer cell line MDA-MB-231.The expression of TM9SF2 in triple negative breast cancer cell line MDA-MB-231 and nontumorigenic mammary epithelial cell line MCF-10A were measured by Western blot. MDA-MB-231 cells were treated with siRNA-TM9SF2 to knock-down the expression of TM9SF2. The effect of silencing TM9SF2 was measured with Western blot.The proliferation of cells was tested by MTS,and the migration was measured with Transwell and wound-healing assay.Proteins related to proliferation (PI3K,AKT,SRC and ERK) and migration (Snail,Slug and N-cadherin) were measured with Western blot.Protein expressions of TM9SF2 was better improved in triple negative breast cancer MDA-MB-231 cell line than MCF-10A.Compared with the control group, the siRNA-TM9SF2 infected group had lower expressions of PI3K, Snail, Slug and N-cadherin, and at the same time phosphorylation of AKT was decreased. The results suggest TM9SF2 can promote the proliferation and metastasis of triple negative breast cancer MDA-MB-231 cell line.

     

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