药物制剂分光光度分析中消除干扰吸收的研究 Ⅱ.一阶导数光谱法的应用—常药降压片中氢氯噻嗪的含量测定

Studies on the elimination of interfering absorption in spectrophotometry of pharmaceutical preparations Ⅱ.The application of first-derivative spectrometry to the determination of dihydrochlorothiazide in hypotensive tablets.

摘要: 导数光谱法是解决可见——紫外光谱干扰的新方法。该法原理简单、操作方便。本文分别在附有导数装置的 UV-300型自记式分光光度计及普通的手动式751G 型分光光度计上用一阶导数法测定了常药降压片中氢氯噻嗪的含量。其回收率分别为99.51±0.47%及99.14±0.45%(置信率95%)。

Abstract: The derivative spectrometry is a new technique eliminating the interference in ultraviolet-visible spectra. This method is very simple and convenient to operate. This paper deals with the determination of dihydrochlorothiazide in hypotentive tablets by the first-derivative spectrometry using a recording double beam spectrophotometer with an attachment (UV-300) or a manual pectrophotometer (751G).The mean recoveries of dihydrochlorothiazide were found to be 99.51±0.47% and 99.14±0.45%( at the 95% confidence level), respectively.