三波长分光光度法测定复方磺胺片中SMPZ和TMP的含量

THREE WAVELENGTH SPECTROPHOTOMETRY OF SMPZ AND TMP IN SULFA COMPOUND TABLETS

摘要: 本文采用三波长分光光度法不经分离直接测定复方磺胺甲氧吡嗪片中磺胺甲氧吡嗪(SMPZ)和甲氧苄氨嘧啶(TMP)的含量。精密称取适量样品,加0.1N HCl振摇,加热,冷却,过滤,取续滤液在精选波长229nm、304nm处测定。SMPZ的平均回收率为99.6±0.29％,TMP的平均回收率为98.7±0.28％。与双波长法、正交函数法相比,本法对复方磺胺甲氧吡嗪片测定的准确度和精密度并无显著性差别。在干扰组分光谱无适当的等吸收点的情况下,应用本法可解决双波长法无法解决的二组分混合物的测定。

Abstract: Sulfamethoxypyrazine (SMPZ) and trimethroprim (TMP) in sulfamethoxypyrazine compound tablets were determined with three wavelength spectrophotometry without any preliminatry separation. The compound tablets were ground and dild. with 0.1 N HCl at 70℃, and analyzed with 751 G spectrophotometer at 229 nm, 304 nm. Recoveries were 99.65±0.31％ and 98.73±0.31% respectively.