反相高效液相色谱溶剂系统优化的正交设计法

SOLVENT OPTIMIZATION OF REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC SEPARATION BY ORTHOGONAL DESIGN

摘要: 在高效液相色谱(HPLC)中,通过改变流动相组成来调节色谱系统的选择性,以适应各种分析要求,但如何改变流动相组成以适应分析要求往往是一项费时而又复杂的工作。目前,国内外已报道了许多HPLC分析条件优化的方法,尤其是溶剂系统优化方法。本文

Abstract: A simple rapid procedure for optimization of mobile phase selectivity used in reversed-phase (RP) high performance liquid chromatography (HPLC) was described. The new method of optimization was called orthogonal design. In reversed-phase HPLC separation o