弹性蛋白酶和激肽释放酶的联产工艺
STUDY ON UNITED PRODUCTION OF ELASTASE AND KALLIKREIN
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摘要: 本文探讨了利用阴离子交换树脂直接从J_2树脂分离弹性蛋白酶后的母液中吸附胰激肽释放酶,然后经NH_4Ac洗涤和洗脱及丙酮沉淀而获得胰激肽释放酶的最佳工艺路线。每千克猪胰脏平均可得弹性蛋白酶3.09g(纯度为56u/mg),胰激肽释放酶2.72g(纯度为7.44u/mg).胰激肽释放酶回收率为56.6%。Abstract: A new procedure for isolation of elastase and kallikrein was investigated. The procedure was that elastase was first adsorbed by J_2 resin, the kallikrein in the filtrate was then adsorbed by D resin, both elastase and kallikrein in he resin were eluted b
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