表达芳香族氨基酸转氨酶基因工程菌的细胞固定化初步研究
Primary Studies on the Immobilization of an Engineering E. coli Strain with High Activity of Aromatic Amino Acid Transaminase
-
摘要: 表达芳香族氨基酸转氨酶基因工程菌的细胞固定化初步研究史燕东,吴梧桐,张玉彬,周艳,黄晓风(中国药科大学生物技术中心,南京210009)关键词卡拉胶;聚丙烯酰胺凝胶;明胶-戊二醛;固定化细胞;芳香族氨基酸转氨酶;CTB2pKB7重组质粒由大肠杆菌tyr...Abstract: CTB2,an E. coli strain constructed by recombinant DN A technology, was immobilized by three different materiais:carragheenin, polyacrylamide and gelatine. By comparing the results of the three methods,we get the conclusion that the methed of using polyacr