丙烯酰胺改性毛细管柱性能考察及应用研究

Study of Performance and Application in Modified Capillary Electrophoresis Column

摘要: 使用丙烯酰胺改性毛细管柱，生物大分子样品柱效达１２５０００／ｍ，小分子样品柱效达１７００００／ｍ。峰形，选择性均较未改性柱有较大改善；连续５次操作迁移时间的相对标准偏差＜２％。在ｐＨ＜７下进样２８２次，性能未见明显降低。该改性柱可以用于拆分手性样品，并能对较复杂样品，如银环蛇毒素样品及中药提取液进行定性分析。

Abstract: A Capillary electrophoresis column was modified by acrylamide for the analysis of small molecule drugs and complicated biological samples. By comparison with the uncoated column, it showed higher efficiency and selectivity. Efficiency as high as 100,000