Abstract: The automatic analysis of bioactive amino acids, the allelochemicals from the whole plants and seeds of Agrostemma githago L. which have the effect of increasing the production of wheat, was carried out. The results indicated that there are 19 kinds of amino acids in seeds with highest content of phenylalanine; while 17 kinds o f amino acids were found in the whole plant, and the content of proline is the highest. A primary realtionship between the ratio of different amino acids and the effect of increasing the production of wheat was discussed.