衍生化寡糖的HPLC/ESI-MS分离和序列分析
HPLC/ESI-MS Analysis of Oligosaccharides as 1-Phenyl-3-methyl-5-pyrazolone Derivatives
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摘要: 采用麦芽糖、麦芽三糖至麦芽七糖作为标准化合物，1－苯基－3－甲基－5－吡咯唑酮衍生化，HPLC／ESI－MS分离及结构分析。结果表明反相液相色谱能将该寡糖衍生物较好地分离；ESI响应强，准分子离子峰为基峰；源内碰撞诱导解离质谱图上有寡糖的序列信息。实验为进一步分析生物体内的寡糖提供了研究方法。Abstract: The 1-phenyl- 3- methyl- 5- pyrazolone derivatives of reducing oligosaccharides, maltose to maltoheptaose, were investigated using HPLC/ESLMS. The derivatives could be well separated in ODS column. It also gave good response signals in the online electros
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