纳米金标记法在DNA检测及基因芯片技术中的应用

Applications of Au Nanoparticles Labeling Method in DNA-detection and the Technology of Genechip

摘要: 近年来DNA介导的纳米金颗粒的组装研究受到广过重视，烷疏基修饰的寡核苷酸可与纳米金共价结合，形成的探针可与目的多核苷酸序列杂交，纳米金颗粒被可逆组装成超分子结构，聚集过程伴有红色到蓝色的颜色变化，由此衍生出比色法检测特定DNA序列的新方法。该方法具有极高的选择性和较好的灵敏度，与银染法结合更提高了检测的灵敏度，可用于斑点亲交、芯片检列等领域。本文对该方法的原理、操作过程及研究进展作一简要介绍。

Abstract: Recently, the research in the DNA mediated assembling of Au nanopartic les has caused wide attention. Mercaptoalkyloligonucleotides c an be covalently bound to Au nanoparticles, which form probes. When these probes were hybridized with th e target poly