RP-HPLC法测定血浆中异烟肼代谢物的含量

RP-HPLC法测定血浆中异烟肼代谢物的含量[J]. 中国药科大学学报, 2004, (1): 49-51.

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RP-HPLC法测定血浆中异烟肼代谢物的含量[J]. 中国药科大学学报, 2004, (1): 49-51.

Determination of Hydrazine Metabolized from Isoniazid in Plasma by RP-HPLC[J]. Journal of China Pharmaceutical University, 2004, (1): 49-51.

Citation:

Determination of Hydrazine Metabolized from Isoniazid in Plasma by RP-HPLC[J]. Journal of China Pharmaceutical University, 2004, (1): 49-51.

Determination of Hydrazine Metabolized from Isoniazid in Plasma by RP-HPLC

摘要: 目的:建立RP-HPLC法测定血浆中异烟肼毒性代谢物肼的方法.方法:采用反相C18柱,以5mmol/L醋酸钠(用1 mol/L醋酸调节pH至5.0)-乙腈(35:65)为流动相,检测波长300nm.结果:肼血浆浓度在8.5～85.2μmol/L范围内线形关系良好(r=0.999),平均回收率为100.2%,日内、日间RSD均小于2%.结论:本方法准确并较为简便,为测定肼血浓以监测异烟肼毒性提供了实验方法.

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