两性霉素B/聚乙二醇-聚谷氨酸苄酯纳米球溶血毒性的研究

Haemolytic Evaluation of Amphotericin B Loaded PEG-PBLG Nanospheres

摘要: 目的:考察两性霉素B/聚乙二醇-聚谷氨酸(AmB/PEG-PBLG)纳米球是否能降低AmB的溶血毒性.方法:超微透析法制备AmB/PEG-PBLG纳米药球,用紫外分光光度法测定样品的溶血率.结果:AmB/PEG-PBLG纳米药球平均粒径142.1 nm,载药量27.55%;溶血率从低到高依次为:PEG-PBLG空白纳米球、脱氧胆酸钠、AmB/PEG-PBLG纳米药球、AmB注射液.结论:AmB/PEG-PBLG纳米药球能有效降低AmB的溶血毒性.

Abstract: AIM:To investigate whether the amphotericin B (AmB) loaded PEG-PBLG nanospheres decreased the hemolytic toxicity.METHOD:AmB loaded PEG-PBLG nanospheres were prepared by diafiltration method.The haemolytic percentage was measured by UV.RESULT:The mean part