神经再生素对PC12细胞Caspases-3的影响

Effect of Nerve Regeneration Factor on Caspases-3 of PC12 Cells

摘要: 目的:探讨神经再生素抑制PC12细胞凋亡的作用机制.方法:采用流式细胞仪和RT-PCR,观察神经再生素对无血清状态下PC12细胞Caspases-3活力及Bax、Caspases-3 mRNA的影响.结果:与对照组相比,神经再生素可抑制无血清培养的PC12 细胞Caspases-3活力,并可使Bax、Caspases-3 mRNA的表达减少.结论:神经再生素可以通过抑制Caspases-3基因表达和活化,而发挥其抗凋亡作用.

Abstract: AIM:To study the mechanism of nerve regeneration factor (NRF) on apoptosis of PC12 cells. METHOD: The activity of Caspases-3 and the expression of Bax,Caspases-3 mRNA of PC12 cells were detected by FCM and RT-PCR under serum free condition.RESULT: The a