Abstract: Aim ： To observe the influence of high fat-high sucrose diet and chronic stress on insulin resistance. Methods ：Male rats were divided into four groups：control，high fat-high sucrose diet(HFSD)，chronic stress(CS)，high fat-high sucrose diet plus chronic stress(HFSD+CS).After feeding the animals for 10 weeks，fat，glucose and insulin concentrations in blood and PPAR-α mRNA expression in liver were examined，and glucose infusion rate was detected by a hyperinsulinemic euglycemic clamp experiment. Results ：Insulin resistance was observed in all three treated groups，showing the highest in the HFSD+CS group.Dyslipidemia，hyperglycosemia，hyperinsulinism and the decrease of PPAR-α mRNA expression in liver were also shown in all treated groups.There was an obvious interaction of insulin resistance，hyperglycosemia and high FFA between high fat-high sucrose diet and chronic stress. Conclusion ：Combination of high fat-high sucrose diet and chronic stress could promote the development of insulin resistance，which is likely due to the high level of serum FFA.