Abstract: Novel aminopropyl-functionalized periodic mesoporous organosilicas （APMO） were successfully prepared by co-condensation of 1，2-bis （triethoxysilyl） ethane （BTSE） and 3-aminopropyltriethoxysilane （APTES） with the template of cationic surfactants cetyltrimethylammonium chlorine （C₁₈ TAC）in basic medium.With the characterization measurements of powder X-ray diffraction（XRD），FT-IR and scanning electron microscopy （SEM） so on，APMO had such advantages as good monodisperse microshperes，uniformly orderd mesopore and large specific surface areas.These mesoporous materials were directly considered to be the chromatographic stationary phases and analyzed by HPLC evaluation.During the research，the as-prepared APMO column had better permeability and three polycyclic aromatic compounds realized separation.In addition，the as prepared APMO column had certain advantages in fast analysis.