Abstract: The study was to explore the effects of dehydroevodiamine(DHED) and berberine hydrochloride(BH) on learning and memory and anti-oxidative capability in aging model mice induced by D-galactose，and to approach the molecular mechanisms of action.D-galactose(120 mg/kg) was sc injected into mice for 60 days to establish the subacute aging model，and medication groups were ig administered 10 days before animal modeling.Morris water maze testing apparatus was used to determine the ability of mice′s learning and memory；the activity of SOD and the contents of MDA in the brain tissue were detected separately.The results showed that in place navigation test，the escape latency and searching distance of model mice were significantly longer than those of the control group(P<0.05，P<0.05)；and DHED high-dose-treated group had shorter escape latency and searching distance compared with the model group.In space probe test，the control group had longer staying time in original platform quadrant than the model group(P<0.01).DHED-treated groups and BH low dose group could significantly prolong the staying time and distance of the original platform quadrant(P<0.001，P<0.05，P<0.05).Compared with the control group，the SOD activity in the brain of model mice decreased(P<0.05)；the SOD activity of brain were enhanced significantly in high-dose DHED group and low-dose BH group in comparison with the model group(P<0.01，P<0.05).These findings indicate that DHED and BH could improve the ability of learning and memory of model mice induced by D-galactose，and that the former has better effect with its mechanism being related with the ability of anti-oxidative stress.