Abstract: Membrane permeability is one of the key properties which affect drug safety and efficacy,so its evaluation is very important for enhancing drug development efficiency and reducing drug reseach spendings. Recently,in-vitro evaluation methods have become the focus in the field of drug membrane permeability researches. In this paper,the developments of in-vitro evaluation methods are summarized,including two-phase partitioning method,chromatography method,capillary electrophoresis method,optical biosensor assay and parallel artificial membrane assay.