化妆品中糖皮质激素类非法添加物的LC-MS/MS分析

LC-MS/MS determination of glucocorticoids illegally added into cosmetics

摘要: 建立化妆品中醋酸可的松、泼尼松、泼尼松龙、曲安西龙、地塞米松和醋酸地塞米松等非法添加物的LC-MS/MS同时测定方法。化妆品经酸化甲醇（冰醋酸调pH为4.0）超声提取，正己烷除脂净化，HPLC-MS/MS分析，流动相为甲醇-0.1%甲酸（60∶40），色谱柱为岛津Shim-pack VP-ODS C₁₈(150 mm×2.0 mm,5 μm)。方法学试验表明：本方法可作为化妆品中非法添加糖皮质激素的常规分析方法。

Abstract: An analytical method for the simultaneous determination of cortisone acetate,prednisone,hydroprednisone,triamcinolone,dexamethasone and dexamethasone acetate in cosmetics using LC-MS/MS was developed. Cosmetic samples were extracted by acidified methanol and analyzed by LC-MS/MS using an electrospray source. The separation was carried out on a Shimadzu Shim-pack VP-ODS C₁₈ (150 mm×2.0 mm,5 μm) and the mobile phase consisted of methanol-0.1% formic acid solution (60∶40). The established method is suitable for routine monitoring and determination of glucocorticoids added in cosmetics illegally.