抗肿瘤海洋放线菌LYG-1的筛选及分类鉴定

Mechanism of the differentiation of human leukemia cell line HL-60 induced by ursolic acid

摘要: 从连云港海域海泥中分离得到一株具有抗肿瘤活性海洋放线菌LYG-1，通过对其形态特征、培养特征、生理生化特性的研究以及16S rRNA基因序列分析，将海洋放线菌LYG-1归属为链霉菌属玫瑰孢链霉菌（Streptomyces roseosporus）的新的海洋变种。利用MTT法对海洋放线菌LYG-1发酵产物的抗肿瘤活性进行了初步研究，结果表明放线菌LYG-1的发酵原液对HepG2、MCF-7、HCT116以及MDA-MB-231 4种肿瘤细胞具有良好的体外抗肿瘤活性。

Abstract: Marine actinomyces LYG-1 was isolated from marine mud flats in Lianyungang,China.Strain LYG-1 was identified using the methods of morphology,physiological and chemotaxonomic characterization and 16S rRNA gene sequence analysis.The results showed that strain LYG-1 was a marine variable species of Streptomyces roseosporus.The fermentation broth of strain LYG-1 exhibited conspicuous antitumor activity against HepG2,MCF-7,HCT116 and MDA-MB-231 cell lines,and the IC₅₀ values were defined by MTT method respectively.