Anti-miRNA-21增加肝细胞癌Huh 7细胞对吉非替尼的敏感性

彭洪清; 李光照; 向琼

Anti-miRNA-21增加肝细胞癌Huh 7细胞对吉非替尼的敏感性

Anti-miRNA-21 increases sensitivity of hepatocellular carcinoma Huh 7 cells to gefitinib

摘要

摘要: 为了探讨anti-miRNA-21与肝细胞癌Huh 7细胞对吉非替尼敏感性之间的关系，通过MTT法和流式细胞术检测anti-miRNA-21联合吉非替尼对Huh 7细胞增殖和凋亡的影响。用Western blot检测anti-miRNA-21对同源性磷酸酶-张力蛋白（PTEN）表达的影响，以及联合吉非替尼对ERK/Akt信号通路相关蛋白表达的影响。结果显示，anti-miRNA-21联合吉非替尼可显著抑制Huh 7细胞增殖（P<0.05），并促进其凋亡；anti-miRNA-21可以上调Huh 7细胞中PTEN的表达；miRNA-21联合吉非替尼对ERK/Akt信号通路的抑制高于吉非替尼组。结果表明,anti-miRNA-21可以增加肝细胞癌Huh 7细胞对吉非替尼的敏感性。

Abstract: In order to investigate the effect of anti-miRNA-21 on the sensitivity of hepatocellular carcinoma cell lines Huh 7 to gefitinib,MTT assay and flow cytometry were used to detected the proliferation and apoptosis of Huh 7 cells transfected by anti-miRNA-21 on the induction of gefitinib.Western blot was used to detect the expression of phosphatase and tensin homolog(PTEN) and relative protein of ERK/Akt pathway.The results showed that anti-miRNA-21 combining with gefitinib inhibit the proliferation of Huh 7 cells significantly (P<0.05)；and inhibited the expression of ERK/Akt pathway related protein more efficiently.Anti-miRNA-21 could upregulate the expression of PTEN in Huh 7 cells.In conclusion,anti-miRNA-21 increased the sensitivity of hepatocellular carcinoma Huh 7 cells to gefitinib.

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