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房长进, 宋敏, 杭太俊, 张晓磊, 苏浩明, 姜翠翠. 二妙丸中3种生物碱成分在Beagle犬体内的药代动力学[J]. 中国药科大学学报, 2012, 43(5): 438-442.
引用本文: 房长进, 宋敏, 杭太俊, 张晓磊, 苏浩明, 姜翠翠. 二妙丸中3种生物碱成分在Beagle犬体内的药代动力学[J]. 中国药科大学学报, 2012, 43(5): 438-442.
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FANG Chang-jin, SONG Min, HANG Tai-jun, ZHANG Xiao-lei, SU Hao-ming, JIANG Cui-cui. Pharmacokinetics of berberine,jatrorrhizine and palmatine in Beagle dogs after oral administration of Ermiao Wan[J]. Journal of China Pharmaceutical University, 2012, 43(5): 438-442.
Citation: FANG Chang-jin, SONG Min, HANG Tai-jun, ZHANG Xiao-lei, SU Hao-ming, JIANG Cui-cui. Pharmacokinetics of berberine,jatrorrhizine and palmatine in Beagle dogs after oral administration of Ermiao Wan[J]. Journal of China Pharmaceutical University, 2012, 43(5): 438-442.
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二妙丸中3种生物碱成分在Beagle犬体内的药代动力学

Pharmacokinetics of berberine,jatrorrhizine and palmatine in Beagle dogs after oral administration of Ermiao Wan

    摘要
  • 摘要: 建立高灵敏的LC-MS/MS法测定血浆中小檗碱、巴马汀和药根碱的浓度，并用于研究Beagle犬灌服市售二妙丸后体内3种生物碱的药代动力学特征。6只健康Beagle犬，雌雄各半，单剂量灌服市售二妙丸制剂(相当于小檗碱25 mg/kg)后，0~48 h间隔采集血样，采用电喷雾正离子化质谱法监测血浆中各成分的浓度。测得小檗碱、药根碱和巴马汀的cmax分别为(4.474±1.8)，(0.508 3±0.2)，(0.812 4±0.7)ng/mL，t1/2分别为(38.1±15.4)，(23.8±9.9)，(20.2±17.1)h，AUC0-48 h分别为(37.4±3.9)，(3.75±1.2)，(3.01±0.9)ng·h/mL。结果显示Beagle犬口服给药二妙丸后小檗碱、药根碱和巴马汀的血药浓度低，达峰时间快，消除半衰期长。

     

    Abstract: This study developed an LC-MS/MS method for the simultaneous determination and plasma pharmacokinetic study of berberine,jatrorrhizine and palmatine in beagle dogs after oral administration of Ermiao Wan.Six dogs (3 males and 3 females) were orally administered with Ermiao wan (equal to berberine 25mg/kg).Blood samples were collected into heparinized tubes from 0 to 48 h.The detection was performed by positive electrospray ionization (ESI) in the selected reaction monitoring mode.The main pharmacokinetic parameters of berberine,jatrorrhizine and palmatine were obtained as follows:cmax(4.474±1.8),(0.508 3±0.2) and (0.812 4±0.7) ng/mL,t1/2(38.1±15.4),(23.8±9.9) and (20.2±17.1) h,AUC0-48 h(37.4±3.9),(3.75±1.2) and (3.01±0.9) ng·h/mL.The results indicated that the oral bioavailability of berberine,jatrorrhizine and palmatine were very low.They were absorbed rapidly,and eliminated slowly in vivo.

     

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