PEG修饰对溶菌酶体外折叠过程的影响

基金项目: 中央高校基本科研业务费专项基金资助项目(No.JKQ2011043);国家基础科学人才培养基金资助项目(No.J0630858);江苏省“青蓝工程”资助项目

Effect of PEGylation on the refolding of lysozyme

摘要: 以溶菌酶为模型蛋白，通过活性测定比较了PEG修饰前后溶菌酶复性动力学的差异，同时利用圆二色谱、色氨酸荧光谱等方法比较了PEG修饰前后蛋白质折叠过程中构象变化。结果显示：PEG修饰改变了溶菌酶复性动力学过程，使溶菌酶在低浓度变性剂条件下快速折叠。色氨酸荧光谱结果显示，PEG修饰对溶菌酶折叠过程中疏水微环境的形成有明显影响，稳定了溶菌酶折叠过程中的“熔球态”，从而提高了溶菌酶正确折叠的概率。
- PEG修饰 /
- 蛋白质体外折叠 /
- 熔球态 /
- 荧光光谱 /
- 圆二色谱
Abstract: The dynamic process of PEGylated lysozyme and native lysozyme refolding were studied The dynamic process of PEGylated lysozyme and native lysozyme refolding were studied by circular dichroism and fluorescence spectrum.Results showed that PEGylation could change lysozyme refolding kinetic process,and make lysozyme refold at lower denaturing agent concentration.Fluorescence spectrum analysis showed that PEGylation has obvious effect on formation of hydrophobic microenvironment during lysozyme refolding process.PEGylation stabilizes the “molten globule” of lysozyme in refolding process,thus increasing the ratio of right refolding of lysozyme.
- PEGylation /
- protein refolding /
- molten globule /
- fluorescence spectrum /
- circular dichroism