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三维肿瘤球模型应用于肿瘤耐药机制研究进展

郭雷雷, 徐郁蕊, 张蕾, 董怡文, 林舒婷, 宁兴海, 刘潇璇

郭雷雷, 徐郁蕊, 张蕾, 董怡文, 林舒婷, 宁兴海, 刘潇璇. 三维肿瘤球模型应用于肿瘤耐药机制研究进展[J]. 中国药科大学学报, 2018, 49(5): 521-527. DOI: 10.11665/j.issn.1000-5048.20180502
引用本文: 郭雷雷, 徐郁蕊, 张蕾, 董怡文, 林舒婷, 宁兴海, 刘潇璇. 三维肿瘤球模型应用于肿瘤耐药机制研究进展[J]. 中国药科大学学报, 2018, 49(5): 521-527. DOI: 10.11665/j.issn.1000-5048.20180502
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GUO Leilei, XU Yurui, ZHANG Lei, DONG Yiwen, LIN Shuting, NING Xinghai, LIU Xiaoxuan. Advances in the application of three-dimensional tumor spheroids model in the mechanism study of drug resistance[J]. Journal of China Pharmaceutical University, 2018, 49(5): 521-527. DOI: 10.11665/j.issn.1000-5048.20180502
Citation: GUO Leilei, XU Yurui, ZHANG Lei, DONG Yiwen, LIN Shuting, NING Xinghai, LIU Xiaoxuan. Advances in the application of three-dimensional tumor spheroids model in the mechanism study of drug resistance[J]. Journal of China Pharmaceutical University, 2018, 49(5): 521-527. DOI: 10.11665/j.issn.1000-5048.20180502
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三维肿瘤球模型应用于肿瘤耐药机制研究进展

  • 1.

    中国药科大学天然药物活性组分与药效国家重点实验室

  • 2.

    中国药科大学药物科学研究院高端药物制剂与材料研究中心

  • 3.

    南京大学现代工程与应用科学学院

基金项目: 国家自然科学基金资助项目(No.51773227,No.81701815,No.21472090)；江苏省自然科学基金资助项目(No.BK20170735)；中组部“千人计划”青年项目；江苏省“高层次创新创业人才引进计划”资助项目；江苏省六大人才高峰计划资助项目；中国药科大学天然药物活性组分与药效国家重点实验室资助项目(No.SKLNMZZRC201804,No.SKLNMKF201703)

Advances in the application of three-dimensional tumor spheroids model in the mechanism study of drug resistance

摘要

    摘要
  • 摘要: 相比于传统的二维肿瘤细胞培养，三维肿瘤球技术不仅为肿瘤细胞提供与体内相似的生长环境，而且还能最大程度地维持肿瘤细胞的生理功能，因此广泛地应用于肿瘤学研究。特别是三维肿瘤球保留了体内细胞所处微环境的物质及结构基础，更接近于实际的病理生理环境，提高了肿瘤细胞对药物的耐受性，是评价肿瘤治疗手段和肿瘤耐药研究的理想体外模型。本文重点综述了三维肿瘤球形态及微环境诱导产生耐药机制方面的研究，并提出现阶段三维肿瘤球模型存在的问题及今后的发展方向。
    Abstract: In comparison with the traditional two-dimensional tumor cell culture, the three-dimensional tumor spheroid culture can not only provide with an in vivo-like growth environment for tumor cells, but also maintain maximum cell activities. Therefore, the three-dimensional tumor spheroid culture is widely used in oncology research. In particular, the three-dimensional tumor cells retain the material and structural basis of the microenvironment of tumor in vivo, which is closer to the actual physiological environment, allowing it to be an ideal in vitro model for evaluating the tumor treatment response and drug resistance in tumors. This review summarizes the mechanisms of drug resistance in three-dimensional tumor cells, especially those induced by the morphology and microenvironment of three-dimensional tumor spheres, and puts forward the problems existing in the current three-dimensional tumor cells model, as well as the future development direction.
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  • 刊出日期:  2018-10-24

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