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LIU Wanwan, ZHANG Shengyu, YANG Yu, ZHU Hongyan. Preparation of tumor-targeting chitosan micelle with transmembrane effect[J]. Journal of China Pharmaceutical University, 2015, 46(5): 561-567. DOI: 10.11665/j.issn.1000-5048.20150508
Citation: LIU Wanwan, ZHANG Shengyu, YANG Yu, ZHU Hongyan. Preparation of tumor-targeting chitosan micelle with transmembrane effect[J]. Journal of China Pharmaceutical University, 2015, 46(5): 561-567. DOI: 10.11665/j.issn.1000-5048.20150508
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Preparation of tumor-targeting chitosan micelle with transmembrane effect

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  • Chitosan(CS)surface was modified with hydrophobic octyl groups to prepare N-octyl chitosan(nitrogen-octyl chitosan, OC). Then hydrophilic group carboxyl-polyethylene glycol-amino(PEG), tumor-targeting ligand D-glucosamine(DG), and membrane-penetrating peptide 9-D-arginine(9R)were linked to OC successively. Then the DG and 9R modified chitosan micelle(DG/9R-PEG-OC)with tumor-targeting and transmembrane effect was prepared. By hydrogen nuclear magnetic resonance spectrometer(1H NMR)and sodium dodecyl sulfate polyacryl amide gel electrophoresis(SDS-PAGE), the successful formation of DG/9R-PEG-OC was certified, with particle size of 151. 8 nm and Zeta potential of 16. 5 mV. The morphology of chitosan micelle observed by transmission electron microscope was homogeneous spherical structure. The drug loading content(DLC)(using fluorescein as a model drug)and encapsulation efficiency(EE)were about 28. 2% and 75. 0% measured by UV-visible spectrophotometer. Meanwhile, the drug showed a controlled releasing profile out of the micelle. Cellular uptake experiments indicated DG/9R-PEG-OC micelle had a significant tumor-tageting and transmembrane effects, especially on HepG2 cells, which exbihited high expression of the glucose transporter. Thus DG/9R-PEG-OC micelle could be a promising drug targeted delivery system of hydrophobic antitumor drugs.
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  • [1]
    Brijesh KS,Sirohi R,Archana D,et al.Porous chitosan scaffolds:a systematic study for choice of crosslinker and growth factor incorporation[J].Int J Polymer Mater,2015,5(64):242-252.
    [2]
    Zhang L,Zhang N.Advances of chitosan and its derivatives in drug delivery system[J].Chin J New Drugs Clin Rem(中国新药与临床志),2014,33(1):9-14.
    [3]
    Hu HY,Zhu H.Adsorption of heavy metal ions by chitosan and its derivatives[J].Prog Chem(化学进展),2012,24(11):2212-2223.
    [4]
    Li SW,Tian JM,Jin J,et al.Targeted biodistribution of aminoglucose-modified doxorubicin.[J].J China Pharm Univ(中国药科大学学报),2012,43(3):266-270.
    [5]
    Nijsten MW,van Dam GM.Hypothesis:using the Warburg effect against cancer by reducing glucose and providing lactate[J].Med Hypotheses,2009,73(1):48-51.
    [6]
    Ganapathy V,Thangaraju M,Prasad PD.Nutrient transporters in cancer:relevance to Warburg hypothesis and beyond[J].Pharmacol Ther,2009,121(1):29-40.
    [7]
    Chen J,Chen HY,Cui SS,et al.Glucosamine derivative modified nano structured lipid carriers for targeted tumor delivery[J].J Mater Chem,2012,22:5770-5783.
    [8]
    Lindgren M,Hällbrink M,Prochiantz A,et al.Cell-penetrating peptides[J].Trends Pharmacol Sci,2000,21(3):99-103.
    [9]
    Meloni BP,Milani D,Edwards AB,et al.Neuroprotective peptides fused to arginine-rich cell penetrating peptides:neuroprotective mechanism likely mediated by peptide endocytic properties[J].Pharmacol Ther,2015,153:36-54.
    [10]
    Yin HQ,Zhang YH,Wang H,et al.The expression of fusion protein Myc-R9-EGFP and identification of its transmembrane ability[J].Biomed Eng(生物医学工程学杂志),2012,29(3):508-513.
    [11]
    Wei YF.The application of cell-penetrating peptide in algae cells transduing allogenic material[D].Shandong:Graduate School of Chinese Academy of Sciences(Marine Research Institute):2014.
    [12]
    Zhang N,Bai Y,Zhao JZ,et al.Preparation and penetrating effect of the polyarginine enhanced green fluorescence protein fusion protein[J].Chi J Biotechnol(生物工程学报),2013,29(11):1644-1653.
    [13]
    Nagahara H,Vocero-Akbani AM,Snyder EL,et al.Transduction of full-length TAT fusion proteins into mammalian cells:TAT-p27 Kip1 induces cell migration[J].Nat Med,1998,4(12):1449-1452.
    [14]
    Giacomelli C,Schmidt V,Borsali R.Nanocontainers formed by self-assembly of poly(ethylene oxide)-b-poly(glycerol monomethacrylate)-drug conjugates[J].Macromolecules,2007,40:2148-2157.
    [15]
    Morita T,Horikiri Y,Suzuki T,et al.Preparation of gelatin microparticles by co-lyophilization with poly(ethylene glycol):characterization and application to entrapment into biodegradable microspheres[J].Int J Pharm,2001,219(1/2):127-137.
    [16]
    Ding Y,Wang Y,Opoku-Damoah Y,et al.Dual-functional bio-derived nanoparticulates for apoptotic antitumor therapy[J].Biomaterials,2015,72:90-103.
    [17]
    Shan L,Liu M,Wu C,et al.Multi-small molecule conjugations as new targeted delivery carriers for tumor therapy[J].Int J Nanomed,2015,10:5571-5591.
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