• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
LIU Wanwan, ZHANG Shengyu, YANG Yu, ZHU Hongyan. Preparation of tumor-targeting chitosan micelle with transmembrane effect[J]. Journal of China Pharmaceutical University, 2015, 46(5): 561-567. DOI: 10.11665/j.issn.1000-5048.20150508
Citation: LIU Wanwan, ZHANG Shengyu, YANG Yu, ZHU Hongyan. Preparation of tumor-targeting chitosan micelle with transmembrane effect[J]. Journal of China Pharmaceutical University, 2015, 46(5): 561-567. DOI: 10.11665/j.issn.1000-5048.20150508
shu

Preparation of tumor-targeting chitosan micelle with transmembrane effect

More Information
    Graphical Abstract
    • Abstract
  • Chitosan(CS)surface was modified with hydrophobic octyl groups to prepare N-octyl chitosan(nitrogen-octyl chitosan, OC). Then hydrophilic group carboxyl-polyethylene glycol-amino(PEG), tumor-targeting ligand D-glucosamine(DG), and membrane-penetrating peptide 9-D-arginine(9R)were linked to OC successively. Then the DG and 9R modified chitosan micelle(DG/9R-PEG-OC)with tumor-targeting and transmembrane effect was prepared. By hydrogen nuclear magnetic resonance spectrometer(1H NMR)and sodium dodecyl sulfate polyacryl amide gel electrophoresis(SDS-PAGE), the successful formation of DG/9R-PEG-OC was certified, with particle size of 151. 8 nm and Zeta potential of 16. 5 mV. The morphology of chitosan micelle observed by transmission electron microscope was homogeneous spherical structure. The drug loading content(DLC)(using fluorescein as a model drug)and encapsulation efficiency(EE)were about 28. 2% and 75. 0% measured by UV-visible spectrophotometer. Meanwhile, the drug showed a controlled releasing profile out of the micelle. Cellular uptake experiments indicated DG/9R-PEG-OC micelle had a significant tumor-tageting and transmembrane effects, especially on HepG2 cells, which exbihited high expression of the glucose transporter. Thus DG/9R-PEG-OC micelle could be a promising drug targeted delivery system of hydrophobic antitumor drugs.
    • FullText(HTML)
    • References (17)
  • [1]
    Brijesh KS,Sirohi R,Archana D,et al.Porous chitosan scaffolds:a systematic study for choice of crosslinker and growth factor incorporation[J].Int J Polymer Mater,2015,5(64):242-252.
    [2]
    Zhang L,Zhang N.Advances of chitosan and its derivatives in drug delivery system[J].Chin J New Drugs Clin Rem(中国新药与临床志),2014,33(1):9-14.
    [3]
    Hu HY,Zhu H.Adsorption of heavy metal ions by chitosan and its derivatives[J].Prog Chem(化学进展),2012,24(11):2212-2223.
    [4]
    Li SW,Tian JM,Jin J,et al.Targeted biodistribution of aminoglucose-modified doxorubicin.[J].J China Pharm Univ(中国药科大学学报),2012,43(3):266-270.
    [5]
    Nijsten MW,van Dam GM.Hypothesis:using the Warburg effect against cancer by reducing glucose and providing lactate[J].Med Hypotheses,2009,73(1):48-51.
    [6]
    Ganapathy V,Thangaraju M,Prasad PD.Nutrient transporters in cancer:relevance to Warburg hypothesis and beyond[J].Pharmacol Ther,2009,121(1):29-40.
    [7]
    Chen J,Chen HY,Cui SS,et al.Glucosamine derivative modified nano structured lipid carriers for targeted tumor delivery[J].J Mater Chem,2012,22:5770-5783.
    [8]
    Lindgren M,Hällbrink M,Prochiantz A,et al.Cell-penetrating peptides[J].Trends Pharmacol Sci,2000,21(3):99-103.
    [9]
    Meloni BP,Milani D,Edwards AB,et al.Neuroprotective peptides fused to arginine-rich cell penetrating peptides:neuroprotective mechanism likely mediated by peptide endocytic properties[J].Pharmacol Ther,2015,153:36-54.
    [10]
    Yin HQ,Zhang YH,Wang H,et al.The expression of fusion protein Myc-R9-EGFP and identification of its transmembrane ability[J].Biomed Eng(生物医学工程学杂志),2012,29(3):508-513.
    [11]
    Wei YF.The application of cell-penetrating peptide in algae cells transduing allogenic material[D].Shandong:Graduate School of Chinese Academy of Sciences(Marine Research Institute):2014.
    [12]
    Zhang N,Bai Y,Zhao JZ,et al.Preparation and penetrating effect of the polyarginine enhanced green fluorescence protein fusion protein[J].Chi J Biotechnol(生物工程学报),2013,29(11):1644-1653.
    [13]
    Nagahara H,Vocero-Akbani AM,Snyder EL,et al.Transduction of full-length TAT fusion proteins into mammalian cells:TAT-p27 Kip1 induces cell migration[J].Nat Med,1998,4(12):1449-1452.
    [14]
    Giacomelli C,Schmidt V,Borsali R.Nanocontainers formed by self-assembly of poly(ethylene oxide)-b-poly(glycerol monomethacrylate)-drug conjugates[J].Macromolecules,2007,40:2148-2157.
    [15]
    Morita T,Horikiri Y,Suzuki T,et al.Preparation of gelatin microparticles by co-lyophilization with poly(ethylene glycol):characterization and application to entrapment into biodegradable microspheres[J].Int J Pharm,2001,219(1/2):127-137.
    [16]
    Ding Y,Wang Y,Opoku-Damoah Y,et al.Dual-functional bio-derived nanoparticulates for apoptotic antitumor therapy[J].Biomaterials,2015,72:90-103.
    [17]
    Shan L,Liu M,Wu C,et al.Multi-small molecule conjugations as new targeted delivery carriers for tumor therapy[J].Int J Nanomed,2015,10:5571-5591.
    • Related Articles

  • Related Articles

    [1]ZHANG Xue, OUYANG Huizhi, XU Wei, WANG Xiaoying. Effect of celastrol combined medication on the inhibition of HepG2 cell proliferation[J]. Journal of China Pharmaceutical University, 2020, 51(2): 185-192. DOI: 10.11665/j.issn.1000-5048.20200209
    [2]WAN Nanyan, JIANG Cuihua, GAO Meng, ZHANG Jian, YIN Zhiqi, PAN Ke. Paeoniflorin inhibits programmed cell death-1-ligand 1 expression in HepG2 cells by regulating JAK/STAT3 signal pathway[J]. Journal of China Pharmaceutical University, 2019, 50(2): 213-221. DOI: 10.11665/j.issn.1000-5048.20190213
    [3]PENG Xuehan, XIE Wenmin, LI Ji, YU Feng. Comparative investigation of in vitro antitumor activity of Ganoderma lucidum spore oil[J]. Journal of China Pharmaceutical University, 2019, 50(1): 81-86. DOI: 10.11665/j.issn.1000-5048.20190111
    [4]LIU Jing, HUANG Yanwei, ZHOU Bo, SONG Qingqing. Effect of R-(+)-lipoic acid on growth, proliferation and related mechanism in human HepG2 cells[J]. Journal of China Pharmaceutical University, 2017, 48(3): 348-354. DOI: 10.11665/j.issn.1000-5048.20170316
    [5]LIU Kunmei, GUO Le, XI Tao. Mechanism study on apoptosis of hepatocellular carcinoma HepG2 cells induced by ursolic acid through inhibiting the expression of miR-21[J]. Journal of China Pharmaceutical University, 2015, 46(6): 745-750. DOI: 10.11665/j.issn.1000-5048.20150619
    [6]WANG Lulu, ZHANG Zhichao, WU Susu, SHANG Jing. Effect of quercetin-3-O-β-D-glucuronide on free fatty acid induced steatosis in HepG2 cells[J]. Journal of China Pharmaceutical University, 2015, 46(5): 587-593. DOI: 10.11665/j.issn.1000-5048.20150512
    [7]FENG Quanfu, BI Lei, YAN Xiaojing, YANG Ye, CHEN Weiping. Inhibition of tetramethypyrazine on proliferation of HepG2 cells and its effects on the pathway of mitochondrial apoptosis[J]. Journal of China Pharmaceutical University, 2015, 46(3): 350-354. DOI: 10.11665/j.issn.1000-5048.20150315
    [8]LIU Zhi-yong, NIU Zhi-yuan, ZHENG Wei, SHEN Ping-ping. Effects of p-ERK1/2 on nitric oxide donor induced apoptosis of HepG2 cells[J]. Journal of China Pharmaceutical University, 2012, 43(6): 530-534.
    [9]Inhibition of glucometabolism by a novel dehydroabielylamine derivative， DHAA-urea，in human hepatoma HepG2 cells[J]. Journal of China Pharmaceutical University, 2010, 41(2): 160-165.
    [10]Effects of the ethanolic extract from Pinellia ternata on cell proliferation and cell cycle of human hepatoma HepG2 cell line[J]. Journal of China Pharmaceutical University, 2009, 40(1): 89-93.

Catalog

    Get Citation
    PDF
    XML
    Article views (1083) PDF downloads (2053) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：416093

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return