Research advances of recombinant coagulation factor VII expression and synthesizing mechanism

Haemophilia is caused by lack of coagulation factor VIII or IX in patients′ blood with inadequate hemostasis. Currently recombinant coagulation factor VII(rFVII)produced in different cells is used against clinical bleeding of haemophilia patients. To enhance the production and activity of rFVII, some eukaryotic cells such as baby hamster kidney(BHK), Chinese hamster ovary(CHO), insect cell and fish embryo, were used to express rFVII. Meanwhile, the effect of functional gene on the activity of rFVII and the limitation of rFVII production caused by post-translational modification were investigated by different methods. The role of rFVII in hemostasis, synthesis of rFVII in different eukaryotic cells and impact on production of post-translational modification are reviewed in this article.