• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
ZHANG Bingfeng, JIANG Hulin. Construction and characterization of mitochondria-targeted TPP-PEI-LND[J]. Journal of China Pharmaceutical University, 2015, 46(6): 659-664. DOI: 10.11665/j.issn.1000-5048.20150604
Citation: ZHANG Bingfeng, JIANG Hulin. Construction and characterization of mitochondria-targeted TPP-PEI-LND[J]. Journal of China Pharmaceutical University, 2015, 46(6): 659-664. DOI: 10.11665/j.issn.1000-5048.20150604

Construction and characterization of mitochondria-targeted TPP-PEI-LND

More Information
  • The mitochondria-targeted TPP-PEI-LND was synthesized by mitochondria-targeted ligand triphenylphosphine(TPP)and therapeutic drug lonidamine(LND)conjugated to low molecular weight branched polyethyleneimine(PEI). TPP-PEI-LND was verified using 1H NMR; in vitro release was determined by the dialysis. Besides, the cytotoxicity and mitochondria-targeted potential of TPP-PEI-LND were investigated in HeLa cells. The results showed that TPP-PEI-LND was successfully synthesized and it exhibited the feature of extended-release. Hence, TPP-PEI-LND could deliver LND to mitochondria, resulting in significantly enhanced efficacy of LND.
  • [1]
    Fulda S,Galluzzi L,Kroemer G.Targeting mitochondria for cancer therapy[J].Nat Rev Drug Discov,2010,9(6):447-464.
    [2]
    Marrache S,Dhar S.Engineering of blended nanoparticle platform for delivery of mitochondria-acting therapeutics[J].Proc Natl Acad Sci U S A,2012,109(40):16288-16293.
    [3]
    Di Cosimo S,Ferretti G,Papaldo P,et al.Lonidamine:efficacy and safety in clinical trials for the treatment of solid tumors[J].Drugs Today(Barc),2003,39(3):157-174.
    [4]
    Li N,Zhang CX,Wang XX,et al.Development of targeting lonidamine liposomes that circumvent drug-resistant cancer by acting on mitochondrial signaling pathways[J].Biomaterials,2013,34(13):3366-3380.
    [5]
    Ringsdorf H.Structure and properties of pharmacologically active polymers[C].J Polym Sci Symp,1975,51(1):135-153.
    [6]
    Hoste K,De Winne K,Schacht E.Polymeric prodrugs[J].Int J Pharm,2004,277(1):119-131.
    [7]
    Cuchelkar V,Kopecková P,Kopecek J.Novel HPMA copolymer-bound constructs for combined tumor and mitochondrial targeting[J].Mol Pharm,2008,5(5):776-786.
    [8]
    Wang M,Hu H,Sun Y,et al.A pH-sensitive gene delivery system based on folic acid-PEG-chitosan-PAMAM-plasmid DNA complexes for cancer cell targeting[J].Biomaterials,2013,34(38):10120-10132.
    [9]
    Roy K,Ghosn B,Kasturi SP.Enhancing polysaccharide-mediated delivery of nucleic acids through functionalization with secondary and tertiary amines[J].Curr Top Med Chem,2008,8(4):331-340.
    [10]
    Nel AE,Mödler L,Velegol D,et al.Understanding biophysicochemical interactions at the nano-bio interface[J].Nat Mater,2009,8(7):543-557.
    [11]
    Mo R,Sun Q,Xue J,et al.Multistage pH-responsive liposomes for mitochondrial-targeted anticancer drug delivery[J].Adv Mater,2012,24(27):3659-3665.
    [12]
    Duncan R,Vicent MJ.Polymer therapeutics-prospects for 21st century:the end of the beginning[J].Adv Drug Deliv Rev,2013,65(1):60-70.
    [13]
    Hu X,Hu J,Tian J,et al.Polyprodrug amphiphiles:hierarchical assemblies for shape-regulated cellular internalization,trafficking,and drug delivery[J].J Am Chem Soc,2013,135(46):17617-17629.
    [14]
    Jiang HL,Kim YK,Arote R,et al.Chitosan-graft-polyethylenimine as a gene carrier[J].J Control Release,2007,117(2):273-280.
    [15]
    Kunath K,von Harpe A,Fischer D,et al.Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery:comparison of physicochemical properties,transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine[J].J Control Release,2003,89(1):113-125.
    [16]
    Hu Q,Gao M,Feng G,et al.Mitochondria-targeted cancer therapy using a light-up probe with aggregation-induced-emission characteristics[J].Angew Chem Int Edit,2014,53(51):14225-14229.
    [17]
    Silvestrini B,Palazzo G,De Gregorio M.Lonidamine and related compounds[J].Prog Med Chem,1984,21:111-135.
    [18]
    Georg IG,Tash JS,Chakrasali R,et al.Lonidamine analogues and treatment of polycystic kidney disease:US,8362031[P].2013-01-29.
  • Related Articles

    [1]TANG Xiaohang, SONG Huilin, YAO Liying, QIN Guowen, WANG Xingchen, LIU Wenyuan, JI Shunli. Determination of non-steroidal anti-inflammatory drugs in the environmental water samples by a polyvinylimide-modified magnetic nanoparticles-based solid phase extraction coupled with high-performance liquid chromatography[J]. Journal of China Pharmaceutical University, 2024, 55(4): 485-492. DOI: 10.11665/j.issn.1000-5048.2023081802
    [2]LI Yanan, WANG Jue, LI Ji, SHEN Yan, TU Jiasheng. Co-delivery of siRNA and docetaxel by hyaluronic acid modified redox-sensitive disulfide-crosslinked nanoparticles[J]. Journal of China Pharmaceutical University, 2016, 47(4): 429-436. DOI: 10.11665/j.issn.1000-5048.20160407
    [3]WU Mingming, FANG Lei, GOU Shaohua, CHEN Li. 以2-甲基-2-取代苯氧基丙酸为离去基团的铂(Ⅱ)配合物的合成、表征及细胞毒活性[J]. Journal of China Pharmaceutical University, 2013, 44(4): 303-306. DOI: 10.11665/j.issn.1000-5048.20130403
    [4]Formula optimization and transfection efficiency of polyethyleneimine/DNA complexes[J]. Journal of China Pharmaceutical University, 2010, 41(1): 40-44.
    [5]Technological Study of N-Chloro-Succimide Synthesis[J]. Journal of China Pharmaceutical University, 2001, (5): 78-79.
    [6]Synthesis of Biodegradable Anhydride-co-imide Terpolymer[J]. Journal of China Pharmaceutical University, 2000, (5): 3-6.
    [10]MECHANISM OF DIFFUSION OF PROGESTERONE THROUGH ETHYLENE VINYL ACETATE COPOLYMER[J]. Journal of China Pharmaceutical University, 1987, (2): 87-90.

Catalog

    Article views (1357) PDF downloads (2514) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return