Advanced Search
CHAI Yingying, CAO Lijuan, ZHANG Haowen, CHEN Hanyu, WANG Guangji, HAO Haiping. Shenmai injection inhibits inflammatory response in lipopolysaccharides-induced septic mice[J]. Journal of China Pharmaceutical University, 2016, 47(1): 79-83. DOI: 10.11665/j.issn.1000-5048.20160111
Citation: CHAI Yingying, CAO Lijuan, ZHANG Haowen, CHEN Hanyu, WANG Guangji, HAO Haiping. Shenmai injection inhibits inflammatory response in lipopolysaccharides-induced septic mice[J]. Journal of China Pharmaceutical University, 2016, 47(1): 79-83. DOI: 10.11665/j.issn.1000-5048.20160111
shu

Shenmai injection inhibits inflammatory response in lipopolysaccharides-induced septic mice

More Information
    Graphical Abstract
    • Abstract
  • This study was to investigate the regulation of lipopolysaccharides(LPS)-induced sepsis in mice by preadministration of Shenmai injection(SMI)and the therapeutic differences between male and female, female and male mice were randomly grouped by weight, including control group, LPS-induced sepsis model group and SMI administration group. After preadministration of SMI for 14 days, 10 mg/kg LPS were intraperitoneally injected subsequently to induce sepsis. The survival rate of mice, level of serum cytokines and the mRNA expression of proinflammatory cytokines in main tissues were detected to evaluate the impact of SMI on LPS-induced sepsis mice. From the survival rate, which is considered as a gold standard of improvement in sepsis, significant protective effect can be observed after SMI pretreatment in LPS-induced sepsis mice, with a more significant effect shown in the females. Consisting with the serum cytokines levels, SMI significantly inhibited proinflammatory cytokines including IL-6, IL-1β and TNF-α mRNA expression in tissues and the regulation of IL-6 was most significant, which was consistent with the results of ELISA in serum. Moreover, the liver tissue acquired a more evident impact than any other tissues, which fits with the ratio of dry/wet weight. SMI can significantly inhibit inflammatory response by delivery in advance in LPS-induced septic mice, which provides strong evidence for elaborating the mechanism in the treatment of cardiovascular disease-related inflammation and shock.
    • FullText(HTML)
    • References (14)
  • [1]
    Bauer A,Bronstrupt M.Industrial natural product chemistry for drug discovery and development[J].Nat Prod Rep,2014,31(1):35-60.
    [2]
    Zhang XD,Ding ZS,Chen JZ.Progress of Shenmai injection in the pharmacological and clinical research[J].Chin J Inf Tradit Chin Med(中国中医药信息杂志),2010,17(3):104-106.
    [3]
    Ganjoo S,Ahmad K,Qureshi,et al.Clinical epidemiology of SIRS and sepsis in newly admitted children[J].Indian J Pediatr,2014,82(8):698-702.
    [4]
    Hotchkiss RS,Monneret G,Payen D.Sepsis-induced immunosuppression:from cellular dysfunctions to immunotherapy[J].Nat Rev Immunol,2013,13(12):862-874.
    [5]
    Wang TS, Deng JC. Molecular and cellular aspects of sepsis-induced immunosuppression[J].J Mol Med(Berl),2008,86(5):495-506.
    [6]
    Shubin NJ,Monaghan SF,Ayala A.Anti-inflammatory mechanisms of sepsis[J].Contrib Microbiol,2011,17:108-124.
    [7]
    Levi M.The coagulant response in sepsis and inflammation[J].Hamostaseologie,2010, 30(1):10-12,14-16.
    [8]
    Yu YH,Cui NQ,Fu Q,et al.Change of TH1/TH2 cytokine equilibrium in rats with severe sepsis and therapeutic effect of recombin Sant interleukin-12 and Shenmai injection[J].Chin J Integr Med,2005,11(2):136-141.
    [9]
    Gao ZL,Yu XQ,Yang M.Clinical trial of treating stress-induced hyperglycemia patients with sepsis by supplementing Qi,nourishing Yin,and promoting blood flow[J].Chin J Intergr Tradit Chin West Med(中国中西医结合杂志),2012,32(10):1336-1339.
    [10]
    Du J,Yang W,Yi D,et al.Exploring real world:analysis about Shenmai injection data extracted from hospital information system database in clinical practice[J].Chin J Intergr Tradit Chin West Med(中国中西医结合杂志),2011,36(20):2813-2816.
    [11]
    Nguyen CT, Luong TT, Kim GL, et al. Korean Red Ginseng inhibits apoptosis in neuroblastoma cells via estrogen receptor beta-mediated phosphatidylinositol-3 kinase/Akt signaling[J].J Ginseng Res,2015,39(1):69-75.
    [12]
    Xu Y, Ding J, Ma XP, et al. Treatment with Panax ginseng antagonizes the estrogen decline in ovariectomized mice[J].Int J Mol Sci,2014,15(5):7827-7840.
    [13]
    Hsu WL,Tsai YT,Wu CT,et al.The prescription pattern of chinese herbal products containing among tamoxifen-treated female breast cancer survivors in Taiwan:a population-based study[J].Evid Based Complement Alternat Med,2015,2015:385204.
    [14]
    Zhong L,Zhou XL,Liu YS,et al.Estrogen receptor alpha mediates the effects of notoginsenoside R1 on endotoxin-induced inflammatory and apoptotic responses in H9c2 cardiomyocytes[J].Mol Med Rep,2015,12(1):119-126.
    • Related Articles

  • Related Articles

    [1]NING Manru, XU Kehui, HANG Taijun, SONG Min. Separation and identification of folic acid and its related substances by liquid chromatography-mass spectrometry[J]. Journal of China Pharmaceutical University, 2025, 56(1): 56-64. DOI: 10.11665/j.issn.1000-5048.2024052201
    [2]XIA Ying, LONG Jiayi, DAI Haoran, SHEN Mengyuan, GUO Hongli, HU Yahui, CHEN Feng. Determination of digoxin in human plasma by LC-MS/MS and its application in pediatric patients[J]. Journal of China Pharmaceutical University, 2021, 52(6): 719-724. DOI: 10.11665/j.issn.1000-5048.20210610
    [3]XIA Ying, GUO Hongli, HU Yahui, CHEN Feng. Therapeutic drug monitoring for monoclonal antibody：progress in the application of LC-MS/MS technique[J]. Journal of China Pharmaceutical University, 2021, 52(1): 122-128. DOI: 10.11665/j.issn.1000-5048.20210117
    [4]NI Yueling, HAN Xing, SONG Min, HANG Taijun. Identification of the related substances of cyclosporin A by LC-MS techniques[J]. Journal of China Pharmaceutical University, 2019, 50(4): 423-437. DOI: 10.11665/j.issn.1000-5048.20190407
    [5]LIANG Fangmei, NI Yueling, WANG Lu, HANG Taijun, SONG Min. Structural identification of the related substances of fusidic acid by LC-MS[J]. Journal of China Pharmaceutical University, 2018, 49(3): 322-332. DOI: 10.11665/j.issn.1000-5048.20180311
    [6]LIU Chaoyi, ZHANG Ge, HANG Taijun, WANG Lei, ZHANG Xiaofei, SONG Min. Identification of related substances in rivaroxaban by LC-MS[J]. Journal of China Pharmaceutical University, 2015, 46(4): 450-457. DOI: 10.11665/j.issn.1000-5048.20150411
    [7]ZHANG Lu, QIANG Shuping, ZHANG Yunjing, WANG Lei, ZHANG Xiaofei, HANG Taijun. Preliminary identification of the related substances in cisatracurium besylate for injection by LC-MS[J]. Journal of China Pharmaceutical University, 2013, 44(1): 65-72. DOI: 10.11665/j.issn.1000-5048.20130111
    [8]KONG Xing-xin, SHEN Wen-bin, WANG Li-yun, SHI Jin-fang, DI Bin, ZHANG Tao. Identification of the related substances in cefmetazole sodium by LC-MS/MS[J]. Journal of China Pharmaceutical University, 2012, 43(2): 170-176.
    [9]QIN Fang, DING Ya, HANG Tai-jun, SONG Min. Identification of the related substances in faropenem sodium by LC-MS/MS[J]. Journal of China Pharmaceutical University, 2011, 42(4): 342-347.
    [10]TIAN Yuan, FENG Shu-dan, HUANG Mei-hua, ZHANG Zun-jian. LC-MS/MS determination of glucocorticoids illegally added into cosmetics[J]. Journal of China Pharmaceutical University, 2011, 42(1): 53-57.

  • Cited by

    Periodical cited type(2)

    1. 王圆超，赵辉，王月，谷岩翡，高月麒，李晓露. 他克莫司的分离提纯研究. 中国抗生素杂志. 2024(02): 175-180 .
    2. 楚士颖，周玉松，张怡铭，唐雨晴，朱思宇，程文播. 液相色谱-串联质谱法同时测定血清中四种免疫抑制剂的药物浓度. 中国当代医药. 2024(09): 7-10+15 .

    Other cited types(0)

Catalog

    Get Citation
    PDF
    XML
    Article views (1026) PDF downloads (1704) Cited by(2)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：309727

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return