Advanced Search
LYU Jiahui, JIANG Minmin, SHI Hui, LI Jianyuan, DU Zhenning. Effects and mechanism of peroxiredoxin-6 on ultraviolet-induced corneal injury in rats[J]. Journal of China Pharmaceutical University, 2016, 47(1): 84-89. DOI: 10.11665/j.issn.1000-5048.20160112
Citation: LYU Jiahui, JIANG Minmin, SHI Hui, LI Jianyuan, DU Zhenning. Effects and mechanism of peroxiredoxin-6 on ultraviolet-induced corneal injury in rats[J]. Journal of China Pharmaceutical University, 2016, 47(1): 84-89. DOI: 10.11665/j.issn.1000-5048.20160112
shu

Effects and mechanism of peroxiredoxin-6 on ultraviolet-induced corneal injury in rats

More Information
    Graphical Abstract
    • Abstract
  • To investigate the therapeutic effect of peroxiredoxin-6(PRDX6)on ultraviolet-induced corneal injury in rats and explore the mechanism, a rat model of corneal injury was established by exposing to ultraviolet. Male Wister rats were randomly divided into control group, dexamethasone(DXM)group and PRDX6 group. All the rats were administered four times a day and for 12 days. The corneal opacity was observed with a slit-lamp microscope. Histopathologic changes were observed with light microscope. The content of corneal malonaldehyde(MDA)was determined by thiobarbituric acid test; the total antioxidative capacity(TAOC)was detected by chemical colorimetric test. P38 MAPK signaling pathway was detected with the method of Western blot and gene expression of cytokines was measured by RT-PCR. Compared with the control group, PRDX6 treatment significantly reduced corneal opacity, improved corneal pathology injury, decreased the MDA content and increased the TAOC. In the PRDX6 group, the level of phosphorylated p38 protein was significantly lower than that in the control group. The gene expression of cytokine was different between the control and PRDX6 groups(P < 0. 05). PRDX6 showed therapeutic effect in the rat model of ultraviolet-induced corneal injury, which may be because it could alleviate the oxidative damage, suppress p38 MAPK phosphorylation and regulate the gene expression of cytokine.
    • FullText(HTML)
    • References (14)
  • [1]
    Youn HY, McCanna DJ, Sivak JG, et al. In vitro ultraviolet-induced damage in human corneal,lens,and retinal pigment epithelial cells[J].Mol Vis,2011,17(3):237-246.
    [2]
    Chandler HL,Reuter KS,Sinnott LT,et al.Prevention of UV-induced damage to the anterior segment using class I UV-absorbing hydrogel contact lenses[J].Invest Ophthalmol Vis Sci,2010,51(1):172-178.
    [3]
    Rubowitz A,Assia EI,Rosner M,et al.Antioxidant protection against corneal damage by free radicals during phacoemulsification[J].Invest Ophthalmol Vis Sci,2003,44(5):1866-1870.
    [4]
    Shi H,Yu HJ,Wang HY,et al.Topical Administration of Peroxiredoxin-6 on the cornea suppresses inflammation and neovascularization induced by ultraviolet radiation[J].Invest Ophthalmol Vis Sci,2012,53(13):8016-8028.
    [5]
    Cui Y,Wu J,Zong M,et al.Proteomic profiling in pancreatic cancer with and without lymph node metastasis[J].Int J Cancer,2009,124(7):1614-1621.
    [6]
    Dickey JB,Cassidy EM,Bouchard CS.Periocular FK-506 delays allograft rejection in rat penetrating keratoplasty[J].Cornea,1993,12(3):204-207.
    [7]
    Erol B,Bozlu M,Hanci V,et al.Coenzyme Q10 treatment reduces lipid peroxidation inducible and endothelial nitric oxide synthases,and germ cell-specific apoptosis in a rat model of testicular ischemia/reperfusion injury[J].Fertil Steril,2010,93(1):280-282.
    [8]
    Suh MH,Kwon JW,Wee WR,et al.Protective effect of ascorbic acid against corneal damage by ultraviolet B irradiation:a pilot study[J].Cornea,2008,27(8):916-922.
    [9]
    Hollyfield JG,Bonilha VL,Rayborn ME,et al.Oxidative damage induced inflammation initiates age-related macular degeneration[J].Nat Med,2008,14(2):194-198.
    [10]
    Yang D, Song Y, Wang X, et al. Deletion of peroxinredoxin 6 potentiates lipopolysaccharide-induced acute lung injury in mice[J].Crit Care Med,2011,39(4):756-764.
    [11]
    Li J,Liu F,Wang H,et al.Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins[J].Mol Cell Proteomics,2010,9(11):2517-2528.
    [12]
    Wu F,Lee S,Schumacher M,et al.Differential gene expression patterns of the developing and adult mouse cornea compared to the lens and tendon[J].Exp Eye Res,2008,87(3):214-225.
    [13]
    Kubo E,Miyazawa T,Fatma N,et al.Development- and age-associated expression pattern of peroxiredoxin 6,and its regulation in murine ocular lens[J].Mech Ageing Dev,2006,127(3):249-256.
    [14]
    Schieven GL.The biology of p38 kinase:a central role ininflammation[J].Curr Top Med Chem,2005,5(10):921-928.
    • Related Articles

  • Related Articles

    [1]NING Manru, XU Kehui, HANG Taijun, SONG Min. Separation and identification of folic acid and its related substances by liquid chromatography-mass spectrometry[J]. Journal of China Pharmaceutical University, 2025, 56(1): 56-64. DOI: 10.11665/j.issn.1000-5048.2024052201
    [2]XIA Ying, LONG Jiayi, DAI Haoran, SHEN Mengyuan, GUO Hongli, HU Yahui, CHEN Feng. Determination of digoxin in human plasma by LC-MS/MS and its application in pediatric patients[J]. Journal of China Pharmaceutical University, 2021, 52(6): 719-724. DOI: 10.11665/j.issn.1000-5048.20210610
    [3]XIA Ying, GUO Hongli, HU Yahui, CHEN Feng. Therapeutic drug monitoring for monoclonal antibody：progress in the application of LC-MS/MS technique[J]. Journal of China Pharmaceutical University, 2021, 52(1): 122-128. DOI: 10.11665/j.issn.1000-5048.20210117
    [4]NI Yueling, HAN Xing, SONG Min, HANG Taijun. Identification of the related substances of cyclosporin A by LC-MS techniques[J]. Journal of China Pharmaceutical University, 2019, 50(4): 423-437. DOI: 10.11665/j.issn.1000-5048.20190407
    [5]LIANG Fangmei, NI Yueling, WANG Lu, HANG Taijun, SONG Min. Structural identification of the related substances of fusidic acid by LC-MS[J]. Journal of China Pharmaceutical University, 2018, 49(3): 322-332. DOI: 10.11665/j.issn.1000-5048.20180311
    [6]LIU Chaoyi, ZHANG Ge, HANG Taijun, WANG Lei, ZHANG Xiaofei, SONG Min. Identification of related substances in rivaroxaban by LC-MS[J]. Journal of China Pharmaceutical University, 2015, 46(4): 450-457. DOI: 10.11665/j.issn.1000-5048.20150411
    [7]ZHANG Lu, QIANG Shuping, ZHANG Yunjing, WANG Lei, ZHANG Xiaofei, HANG Taijun. Preliminary identification of the related substances in cisatracurium besylate for injection by LC-MS[J]. Journal of China Pharmaceutical University, 2013, 44(1): 65-72. DOI: 10.11665/j.issn.1000-5048.20130111
    [8]KONG Xing-xin, SHEN Wen-bin, WANG Li-yun, SHI Jin-fang, DI Bin, ZHANG Tao. Identification of the related substances in cefmetazole sodium by LC-MS/MS[J]. Journal of China Pharmaceutical University, 2012, 43(2): 170-176.
    [9]QIN Fang, DING Ya, HANG Tai-jun, SONG Min. Identification of the related substances in faropenem sodium by LC-MS/MS[J]. Journal of China Pharmaceutical University, 2011, 42(4): 342-347.
    [10]TIAN Yuan, FENG Shu-dan, HUANG Mei-hua, ZHANG Zun-jian. LC-MS/MS determination of glucocorticoids illegally added into cosmetics[J]. Journal of China Pharmaceutical University, 2011, 42(1): 53-57.

  • Cited by

    Periodical cited type(2)

    1. 王圆超，赵辉，王月，谷岩翡，高月麒，李晓露. 他克莫司的分离提纯研究. 中国抗生素杂志. 2024(02): 175-180 .
    2. 楚士颖，周玉松，张怡铭，唐雨晴，朱思宇，程文播. 液相色谱-串联质谱法同时测定血清中四种免疫抑制剂的药物浓度. 中国当代医药. 2024(09): 7-10+15 .

    Other cited types(0)

Catalog

    Get Citation
    PDF
    XML
    Article views (1128) PDF downloads (1793) Cited by(2)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：309727

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return