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WANG Chi, TIAN Hong, GAO Xiangdong, YAO Wenbing. Influence of ubiquitous chromatin opening element on the antibody expression in CHO cells[J]. Journal of China Pharmaceutical University, 2017, 48(1): 96-101. DOI: 10.11665/j.issn.1000-5048.20170115
Citation: WANG Chi, TIAN Hong, GAO Xiangdong, YAO Wenbing. Influence of ubiquitous chromatin opening element on the antibody expression in CHO cells[J]. Journal of China Pharmaceutical University, 2017, 48(1): 96-101. DOI: 10.11665/j.issn.1000-5048.20170115
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Influence of ubiquitous chromatin opening element on the antibody expression in CHO cells

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  • Ubiquitous chromatin opening element(UCOE), composed of the promoters of human housekeeping genes, prevents transgene from silencing and produces consistent, stable and high-level gene expression irrespective of the chromosomal integration site. The research studied the influence of different UCOE element parts on antibody expression in CHO cells. UCOE 1. 5 kb from chromobox homolog 3(CBX3), UCOE 2. 5 kb from the heterogeneous ribonucleoprotein A2/B1(HNRPA2B1)and the whole UCOE 4. 0 kb were inserted into the antibody light and heavy chain vectors, respectively, and transfected into CHO cells using antibiotics-Zeocin and Blasticidin for pressure selection. Four groups of monoclonal cells were harvested and antibody expression of each group was detected. The monoclonal cells with UCOE 1. 5 kb and UCOE 2. 5 kb increased 1. 5 to 2-fold in the level of antibody expression, wheareas, monoclonal cells with UCOE 4. 0 kb increased 3 to 4-fold. The enhancement of two housekeeping promoter genes on antibody expression could stack up.
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  • [1]
    Butler M.Animal cell cultures:recent achievements and perspectives in the production of biopharmaceuticals[J].Appl Microbiol Biotechnol,2005,68(3):283-291.
    [2]
    Barnes LM,Moy N,Dickson AJ.Phenotypic variation during cloning procedures:analysis of the growth behavior of clonal cell lines[J].Biotechnol Bioeng,2006,94(3):530-537.
    [3]
    Osterlehner A,Simmeth S,Göpfert U.Promoter methylation and transgene copy numbers predict unstable protein production in recombinant Chinese hamster ovary cell lines[J].Biotechnol Bioeng,2011,108(11):2670-2681.
    [4]
    Yang Y,Mariati,Chusainow J,et al.DNA methylation contributes to loss in productivity of monoclonal antibody-producing CHO cell lines[J].J Biotechnol,2010,147(3/4):180-185.
    [5]
    Zhang F,Frost AR,Blundell MP,et al.A ubiquitous chromatin opening element(UCOE)confers resistance to DNA methylation-mediated silencing of lentiviral vectors[J].Mol Ther,2010,18(9):1640-1649.
    [6]
    Antoniou M,Harland L,Mustoe T,et al.Transgenes encompassing dual-promoter CpG islands from the human TBP and HNRPA2B1 loci are resistant to heterochromatin-mediated silencing[J].Genomics,2003,82(3):269-279.
    [7]
    Nair AR,Jinger X,Hermiston TW.Effect of different UCOE-promoter combinations in creation of engineered cell lines for the production of Factor VIII[J].BMC Res Notes,2011,4:178.
    [8]
    Liu S,Tian H,Wang C,et al.Weakening resistance marker for establishing a process of screening high-producing CHO cell lines[J].J China Pharma Univ(中国药科大学学报),2015,46(5):617-622.
    [9]
    Almo SC,Love JD.Better and faster:improvements and optimization for mammalian recombinant protein production[J].Curr Opin Struct Biol,2014,26:39-43.
    [10]
    Zhu J.Mammalian cell protein expression for biopharmaceutical production[J].Biotechnol Adv,2012,30(5):1158-1170.
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