• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
WEI Dandan, GUO Sheng, SU Shulan, QIAN Dawei, ZHU Zhenhua, SHANG Erxin, GENG Zhongyi, DUAN Jin′ao. Protective effect of okra seed oil on acute gastric ulcer in mice[J]. Journal of China Pharmaceutical University, 2017, 48(3): 334-342. DOI: 10.11665/j.issn.1000-5048.20170314
Citation: WEI Dandan, GUO Sheng, SU Shulan, QIAN Dawei, ZHU Zhenhua, SHANG Erxin, GENG Zhongyi, DUAN Jin′ao. Protective effect of okra seed oil on acute gastric ulcer in mice[J]. Journal of China Pharmaceutical University, 2017, 48(3): 334-342. DOI: 10.11665/j.issn.1000-5048.20170314
shu

Protective effect of okra seed oil on acute gastric ulcer in mice

More Information
    Graphical Abstract
    • Abstract
  • To investigate the protective effect of the seed oil of Abelmoschus esculentus on gastric ulcer, two acute gastric ulcer mice models were established by intragastric administration of aspirin or absolute ethanol, respectively. Clinical index of ulcer area, ulcer index, gastric volume, gastric pH value, free acidity, total acidity, and histopathological assessment were measured to evaluate the injuries of gastric ulcer and the protective effect of okra seed oil. In order to comprehensively uncover the possible underlying mechanism, a series of biochemical assays were also performed, including serum TNF-α, IL-6, IL-10 and Tbil, NO, MPO and SOD in the stomach included. Moreover, the ALT, AST and ALP in the liver of mice were also tested to evaluate the possible hepatic toxicity of the seed oil. The results indicated that the seed oil of A. esculentus exerted protective effect in ethanol-induced gastric ulcer mice by reducing the ulcer area and ulcer index, declining the free and total acidity, and increasing the pH value of gastric content. Histopathological observation showed the gastric mucosa of the acute gastric ulcer mice induced by alcohol was incomplete and severely damaged, with submucosal edema and nuclear pyknosis, as well as glandular structure disappearing, compared with that of normal mice. What′s more, a number of inflammatory cell infiltration occured in the gastric mucosa of alcohol-model mice, with messes of neutrophils, lymphocytes, eosinophils and plasma cells. Okra seed oil could improve the damaged structure of the gastric mucosa and gland caused by ethanol, but could not ameliorate the condensation of nucleus and infiltration of inflammatory cells. Biochemical analysis revealed that the seed oil of A. esculentus could counteract the damage induced by ethanol via decreasing Tbil and TNF-α in serum, decreasing NO and myeloperoxidase, and increasing SOD in stomach. Meanwhile, okra seed oil exhibited protective effect in aspirin-induced gastric ulcer mice by increasing the gastric content pH, and reducing free and total acidity. Compared with the control group, the gastric mucosa of aspirin-model group showed multifocal coagulation necrosis, sheet edema and infiltration of inflammatory cells by histopathological assessment. Compared with the aspirin-model group, the soybean oil group and okra seed oil group could ameliorate the inflammatory cell infiltration. Biochemical analysis revealed that okra seed oil could counteract the injury induced by aspirin via decreasing TNF-α and IL-6, and increasing IL-10 in serum, decreasing NO and MPO and increasing SOD in stomach. In a word, the okra seed oil exerted protective effect on acute gastric ulcer by anti-inflammation, anti-oxidation and hepatocyte protection. The okra seed oil deserves further development and utilization.
    • FullText(HTML)
    • References (18)
  • [1]
    Zhong LX, Gao FG, Xie CM. Relevant factors and preventive measures of recurrence of peptic ulcer[J].Mod Diagn Treat(现代诊断与治疗),2015,26(18):4268-4269.
    [2]
    Suzuki H,Nishizawa T,Tsugawa H,et al.Roles of oxidative stress in stomach disorders[J].J Clin Biochem Nutr,2011,50(1):35-39.
    [3]
    de Oliveira JG,Rossi AFT,Nizato DM,et al.Influence of functional polymorphisms in TNF-α,IL-8,and IL-10 cytokine genes on mRNA expression levels and risk of gastric cancer[J].Tumor Biol,2015,36(12):9159-9170.
    [4]
    Zhan ZG,Li YJ,Zhang Y.Determination of the major nutrients constituents in Hibiscus esculentus seed[J].Acta Nutr Sin(营养学报),2012,34(2):191-192.
    [5]
    Lyu MY,Guo MP.Determination of six biological elements in the fruits of Hibiscus esculentus and their medical value[J].J Jiangxi Col Tradit Chin Med(江西中医学院学报),2001,13(2):72.
    [6]
    Xu M, Zheng HY, Wang W, et al. The anti-fatigue activity of alkaloids from okra seed[J].Jilin Agr(吉林农业),2014,(3):30-32.
    [7]
    Zheng HY,Gao Y,Wang HB.Optimization of extraction process of caffeine from okra seed and evaluation of antioxidative activity[J].Food Sci Technol(食品科技),2016,41(6):230-236.
    [8]
    Li J,Wang W,Sun XH.Analysis of components in volatile oil and fatty acid from the seed of Abelmoschus esculentus[J].Hubei Agr Sci(湖北农业科学),2012,51(5):1006-1008.
    [9]
    Li WF,Hao DJ,Fan T,et al.Protective effect of chelerythrine against ethanol-induced gastric ulcer in mice[J].Chem Biol Interact,2014,208:18-27.
    [10]
    Singh S,Majumdar DK.Evaluation of the gastric antiulcer activity of fixed oil of Ocimum sanctum(Holy Basil)[J].J Ethnopharmacol,1999,65(1):13-19.
    [11]
    Ibrahim BMM,Salama AAA,Abdallah HMI,et al.Study of the protective effects of flaxseed oil on ethanol induced gastric mucosal lesions in non ovariectomized and ovariectomized rats[J].Int J Pharmacol,2016,12(4):329-339.
    [12]
    Donato-Trancoso A,Monte-Alto-Costa A,Romana-Souza B.Olive oil-induced reduction of oxidative damage and inflammation promotes wound healing of pressure ulcers in mice[J].J Dermatol Sci,2016,83(1):60-69.
    [13]
    Alam S,Hussain MS,Reddy MK,et al.Antiulcer and antioxidant potential of Zizyphus jujuba Mill root extract in aspirin and ethanol induced gastric ulcers[J].Int J Phytomedicine,2016,8(2):287-293.
    [14]
    Li W,Huang H,Niu X,et al.Protective effect of tetrahydrocoptisine against ethanol-induced gastric ulcer in mice[J].Toxicol Appl Pharmacol,2013,272(1):21-29.
    [15]
    Li H,Song ZJ,Dai YP,et al.Antioxidative activity of flavonoids from Abrus cantoniensis against ethanol-induced gastric ulcer in mice[J].Planta Med,2015,81(10):784-790.
    [16]
    Cuevas DM,Calderon PEE,Cruz RC,et al.Protective influence of virgin coconut oil against the development of aspirin-and HCl/ethanol-related gastric ulcers in murine models[C]//Proceedings of the DLSU Research Congress,2016,4:FNH-I-06.
    [17]
    Diniz LRL,Vieira CFX,dos Santos EC,et al.Gastroprotective effects of the essential oil of Hyptis crenata Pohl ex Benth.on gastric ulcer models[J].J Ethnopharmacol,2013,149(3):694-700.
    [18]
    Rozza AL,Pellizzon CH.Essential oils from medicinal and aromatic plants:a review of the gastroprotective and ulcer-healing activities[J].Fundam Clin Pharmacol,2013,27(1):51-63.
    • Related Articles

  • Related Articles

    [1]WU Lingxi, DU Yixuan, GAO Xiangdong. Inhibitory effect of IL-27 on the overactivation of microglia[J]. Journal of China Pharmaceutical University, 2024, 55(6): 801-808. DOI: 10.11665/j.issn.1000-5048.2024022702
    [2]CUI Tao, WU Weidang, CI Xiaoyan, LI Wei, GUO Chuanmin, XU Jing, YI Xiulin, LIU Changxiao. Evaluation of tolerance and pharmacodynamics of nano-micelle irinotecan formulation[J]. Journal of China Pharmaceutical University, 2019, 50(2): 175-179. DOI: 10.11665/j.issn.1000-5048.20190207
    [3]YUE Juanjuan, CUI Hanyue, WANG Hui, YE Bin, CHEN Dingding. Protective effects of losartan potassium on 5/6 nephrectomy-induced chronic heart failure in rats[J]. Journal of China Pharmaceutical University, 2016, 47(6): 734-739. DOI: 10.11665/j.issn.1000-5048.20160617
    [4]JIANG Yuqiu, TANG Qiaofei, ZHANG Shuang, YAN Zhiyong. Combined effects of gallic acid and ciprofloxacin on the murine chronic rhinosinusitis model in mice[J]. Journal of China Pharmaceutical University, 2015, 46(5): 600-604. DOI: 10.11665/j.issn.1000-5048.20150514
    [5]CHEN Lu, LI Jiajie, PAN Cailong, ZHOU Danli, LIU Wentao, ZHANG Guangqin. Tetramethylpyrazine attenuates morphine tolerance through suppressing spinal microglia activation in mice[J]. Journal of China Pharmaceutical University, 2015, 46(2): 230-234. DOI: 10.11665/j.issn.1000-5048.20150216
    [6]WANG Yu, ZHAO Li, MIAO Hanchi, LI Zhiyu, GUO Qinglong. Apoptosis induction of oroxylin A in human chronic myelogenous leukemia cell line K562[J]. Journal of China Pharmaceutical University, 2014, 45(2): 232-236. DOI: 10.11665/j.issn.1000-5048.20140218
    [7]DENG Danni, TIAN Hong, YAO Wenbing. Advances in therapeutic B-cell vaccines against chronic diseases[J]. Journal of China Pharmaceutical University, 2013, 44(5): 470-475. DOI: 10.11665/j.issn.1000-5048.20130517
    [8]FENG Lan-ying, HUANG Wen-ye. Expression and regulation of Bim protein in chronic myelogenous leukemia[J]. Journal of China Pharmaceutical University, 2009, 40(6): 571-575.
    [9]Effect of Amygdalin on Chronic Gastritis and Chronic Atrophic Gastritis[J]. Journal of China Pharmaceutical University, 2002, (1): 47-49.
    [10]Study on the Tolerance of the Rabbit Body Temperature Response to Bacterial Endotoxin[J]. Journal of China Pharmaceutical University, 1999, (3): 64-66.

Catalog

    Get Citation
    PDF
    XML
    Article views (1228) PDF downloads (2163) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：292717

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return