Secondary metabolites of the endophytic fungus <i>Myrothecium</i> sp. from <i>Pinellia ternata</i>

YU Juping; YANG Minghua; HE Xiaorong

doi:10.11665/j.issn.1000-5048.20210405

Journal of China Pharmaceutical University > 2021 > 52(4): 431-437. > DOI: 10.11665/j.issn.1000-5048.20210405

YU Juping, YANG Minghua, HE Xiaorong. Secondary metabolites of the endophytic fungus Myrothecium sp. from Pinellia ternata[J]. Journal of China Pharmaceutical University, 2021, 52(4): 431-437. DOI: 10.11665/j.issn.1000-5048.20210405

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Secondary metabolites of the endophytic fungus Myrothecium sp. from Pinellia ternata

School of Engineering, China Pharmaceutical University, Nanjing 211198, China

Funds: This study was supported by the National Natural Science Foundation of China (No.81773901)

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Received Date: March 29, 2021
Revised Date: July 09, 2021

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Abstract

Abstract

Using a series of purification methods including silica gel， Sephadex LH-20 and preparative high performance liquid chromatography， secondary metabolites of Myrothecium sp. were purified from the ethyl acetate extract of the solid fermentation product. Based on structure characterization and investigation on the physical and chemical properties a， twelve monomeric compounds were identified as 3''-hydroxyverrucarin A (1), verrucarin A (2), verrucarin L acetate (3), verrucarin J (4), verrucarin K (5), roridin A (6), roridin D (7), roridin H (8), roridin J (9), verrol 4-acetate (10), (3S, 3aS, 6α, 6aR)-dihydrosporothrioride (11) and 4,6-dihydroxy-1(3H)-isobenzofuranone (12). Compounds 1, 5 and 9 -12 were isolated from Myrothecium sp. for the first time.Compounds 2, 3, 4, 7 and 8 exhibited strong inhibitory effects on Candida albicans, with a minimal inhibitory concentration （MIC₅₀） of 0.318, 0.218, 0.047, 0.569 and 0.558 μg/mL, respectively.
- Pinellia ternata,
- endophytic fungi,
- Myrothecium sp.,
- verrucarin,
- roridin

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