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YU Zexuan, JU Caoyun, ZHANG Can. Construction and application of gene delivery systems for primary dendritic cells[J]. Journal of China Pharmaceutical University, 2021, 52(4): 438-446. DOI: 10.11665/j.issn.1000-5048.20210406
Citation: YU Zexuan, JU Caoyun, ZHANG Can. Construction and application of gene delivery systems for primary dendritic cells[J]. Journal of China Pharmaceutical University, 2021, 52(4): 438-446. DOI: 10.11665/j.issn.1000-5048.20210406
shu

Construction and application of gene delivery systems for primary dendritic cells

  • Center of Advanced Pharmaceuticals and Biomaterials, China Pharmaceutical University, Nanjing 210009, China

Funds: This study was supported by the National Natural Science Foundation of China (No. 82073785, No.81930099, No.81773664)
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  • Received Date: April 24, 2021
  • Revised Date: June 28, 2021
    Graphical Abstract
    • Abstract
  • Nowadays, there is still no mature gene delivery system for safe and effective transfection on primary dendritic cells (DC). Herein, we constructed a liposome-based gene delivery system for primary DCs and optimized the preparation method to improve the transfection efficiency of siRNA on primary DCs. In this study, different methods, including co-incubation method, ethanol injection method, and protamine compound method, were used to prepare liposome/siRNA complexes based on different cationic lipids. Moreover, particle size, zeta potential, siRNA loading capacity, safety, stability, uptake efficiency and gene silencing efficiency of various liposome/siRNA complexes were detected to screen the optimal cationic lipid as well as its preparation method. We demonstrated that the OA2/siRNA delivery system prepared by the co-incubation method exhibited the best safety, uptake efficiency and gene silencing effect, compared to other siRNA delivery systems including the commercial Lipo2000. In summary, we provide a safe and effective gene delivery vector for primary DC cells through simple preparation method, which could also offer a gene delivery platform for other immune cells.
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    • References (19)
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