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LU Wei-dong, LIN Yi-ju, DAI Yun-bo, YANG Xuan-xiang, MA Bo. Preparation and immunogenicity of influenza vaccine lyophilized liposomes[J]. Journal of China Pharmaceutical University, 2009, 40(3): 218-221.
Citation: LU Wei-dong, LIN Yi-ju, DAI Yun-bo, YANG Xuan-xiang, MA Bo. Preparation and immunogenicity of influenza vaccine lyophilized liposomes[J]. Journal of China Pharmaceutical University, 2009, 40(3): 218-221.

Preparation and immunogenicity of influenza vaccine lyophilized liposomes

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  • Aim :To prepare the influenza vaccine lyophilized liposomes and to characterize its particle distribution,encapsulation efficiency and immunogenicity. Methods :Flu vaccine liposome based on the method of thin-film evaporation was prepared using phospholipids ,cholesterol and the purified influenza virus split vaccine,and was further subjected to frozen-drying.The polymorph was observed by microscope;the particle distribution and the average size were analysed by transmission electron microscope;its encapsulation efficiency was determined by Lowry method and the antibody titers were assessed by hemagglutination-inhibition after pulmonary delivery to mice. Results :The reconstitated influenza vaccine liposome under electronic microscope were round or elliptic particles evenly distributed at a mean size of 2.14 μm,with the encapsulation efficiency of more than 80%.The antibody titer through pulmonary delivery was higher than that through intraperitoneal injection. Conclusion :The prepared influenza vaccine lyophilized liposomes possess high encapsulation efficiency,better particle distribution and marked immunogenicity through pulmonary delivery to mice.Pulmonary delivery of influenza vaccine liposomes is a potential immunization approach worthy of further exploitation.
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