Protective effect and mechanism of<i> Jinshuibao</i> tablet on acute kidney injury induced by cisplatin in rats

LIU Xuewu; ZHOU Zhiming; JIANG Dejian; LONG Li

doi:10.11665/j.issn.1000-5048.20200112

Journal of China Pharmaceutical University > 2020 > 51(1): 76-83. > DOI: 10.11665/j.issn.1000-5048.20200112

LIU Xuewu, ZHOU Zhiming, JIANG Dejian, LONG Li. Protective effect and mechanism of Jinshuibao tablet on acute kidney injury induced by cisplatin in rats[J]. Journal of China Pharmaceutical University, 2020, 51(1): 76-83. DOI: 10.11665/j.issn.1000-5048.20200112

Citation:

PDF (3963 KB)

Protective effect and mechanism of Jinshuibao tablet on acute kidney injury induced by cisplatin in rats

More Information

Graphical Abstract

Abstract

Abstract

This study aimed to observe the therapeutic effect and mechanism of Jinshuibao tablet on acute renal injury induced by cisplatin. Acute renal injury models in SD rats were induced separately by single intraperitoneal injection of cisplatin(5 mg/kg)and intravenous injection for 5 consecutive days at a dosage of 2 mg/kg per day. The renal function and renal histopathological changes were observed in rat acute renal injury models after prevention and treatment with Jinshuibao tablet, respectively. The content of tumor necrosis factor(TNF-α)and reactive oxygen species(ROS), the activity of Caspase 3 and the expression of t-p38, p-p38, Bax and Bcl-2 in the kidneys were detected. The results showed that preventive and therapeutic administration of Jinshuibao tablets could both significantly inhibit the increase of the blood urea nitrogen(BUN)and creatinine(CRE), increase the creatinine clearance rate, reduce the contents of TNF-α and ROS, and decrease the activity of Caspase 3 in acute renal injury models induced by cisplatin. The renal histopathological results showed that Jinshuibao tablets could significantly reduce renal histopathology scores, ameliorate renal tubule degeneration and inflammatory infiltration. Western blot results showed that Jinshuibao tablets could significantly decrease the expression of t-p38 and p-p38, while increasing the Bcl-2/Bax ratio in the kidneys. These results suggested that preventive and therapeutic administration of Jinshuibao tablets could both improve renal function and pathological changes of renal tissue, which might be related to the inhibition of TNF-α and the ROS-p38 MAPK-Caspase3 pathway and thus inhibition of apoptosis.
- Jinshuibao tablets,
- cisplatin,
- acute kidney injury,
- mechanism

FullText(HTML)

References (16)

References

[1]	Chen CJ,Chen CN,Pan QZ,et al.Clinical study on the intervention therapy of Jinshuibao capsule on cisplatins-induced renal toxicity[J].Med Innov China(中国医学创新),2012,9(2):32-33.
[2]	Ye XL,Zhen XC,Yang XL,et al.Diclofenac sodium associated acute kidney injury[J].J ADR(药物不良反应杂志),2018,20(2):146-147.
[3]	Liu W,Jiang SF,Tu L,et al.Mechanism of JinShuiBao capsule in ameliorating rat pneumoconiosis model[J].J China Pharm Univ(中国药科大学学报),2018,49(4):476-482.
[4]	Yan YL,Zhang DM,Wang Y,et al.The comparative study of the effect of intraperitoneal perfusion and intravenous chemotherapy in advanced ovarian cancer[J].J Int Obstet Gynecol(国际妇产科学杂志),2014,41(6):655-657.
[5]	Markman M.Intraperitoneal chemotherapy as primary treatment of advanced ovarian cancer:efficacy,toxicity,and future directions[J].Rev Recent Clin Trials,2007,2(3):169-173.
[6]	Goren O,Matot I.Update on perioperative acute kidney injury[J].Curr Opin Crit Care,2016,22(4):370-378.
[7]	Yan W, Xu Y, Yuan YH, et al. Renoprotective mechanisms of Astragaloside IV in cisplatin-induced acute kidney injury[J].Free Radic Res,2017,51(7/8):669-683.
[8]	Mulay SR,Anders HJ.Crystal nephropathies:mechanisms of crystal-induced kidney injury[J].Nat Rev Nephrol,2017,13(4):226-240.
[9]	Devarajan P.Acute kidney injury:still misunderstood and misdiagnosed[J].Nat Rev Nephrol,2017,13(3):137-138.
[10]	Mansouri A,Ridgway LD,Korapati AL,et al.Sustained activation of JNK/p38 MAPK pathways in response to cisplatin leads to Fas ligand induction and cell death in ovarian carcinoma cells[J].J Biol Chem,2003,278(21):19245-19256.
[11]	He B,Tao HY,Liu SQ,et al.Carboxymethylated chitosan protects rat chondrocytes from NO-induced apoptosis via inhibition of the p38/MAPK signaling pathway[J].Mol Med Rep,2016,13(3):2151-2158.
[12]	Wang JX,Chen HJ,Cao P,et al.Inflammatory cytokines induce caveolin-1/β-catenin signalling in rat nucleus pulposus cell apoptosis through the p38 MAPK pathway[J].Cell Prolif,2016,49(3):362-372.
[13]	Cai QH,Tang Y,Fan SH,et al.In vivo effects of dexmedetomidine on immune function and tumor growth in rats with ovarian cancer through inhibiting the p38MAPK/NF-κB signaling pathway[J].Biomedecine Pharmacother,2017,95:1830-1837.
[14]	Goldsmith EJ,Min XS,He HX,et al.Structural studies of MAP kinase cascade components[J].Methods Mol Biol,2010,661:223-237.
[15]	Sabio G,Davis RJ.TNF and MAP kinase signalling pathways[J].Semin Immunol,2014,26(3):237-245.
[16]	Ashraf M,Ebner M,Wallner C,et al.A p38MAPK/MK2 signaling pathway leading to redox stress,cell death and ischemia/reperfusion injury[J].Cell Commun Signal,2014,12(1):6.

[1]	WU Gelin, FAN Ruixin, LIANG Chuling, XING Leng, XIE Yongjian, GONG Ping, ZHOU Peng, LI Bo. Identification of chemical components and determination of vitexin in the raw powder of Tongluo Shenggu capsule[J]. Journal of China Pharmaceutical University, 2025, 56(2): 166-175. DOI: 10.11665/j.issn.1000-5048.2024090301
[2]	TU Penghuang, YAO Zhiren, JIN Meiling, NING Guanyu, HUANG Yaping, PAN Ke, YIN Zhiqi. Study of glycosides from Piper sintenense Hatusima[J]. Journal of China Pharmaceutical University, 2024, 55(2): 202-208. DOI: 10.11665/j.issn.1000-5048.2023122704
[3]	FANG Siyang, ZHANG Xinggui, YE Yu, WANG Keqing, YAO Zhiren, HUANG Yaping, ZHANG Jian, QIN Yanhua, YIN Zhiqi. A new ester glycoside compound from Cyclocarya paliurus[J]. Journal of China Pharmaceutical University, 2023, 54(1): 56-61. DOI: 10.11665/j.issn.1000-5048.20221209002
[4]	CHENG Xiaohong, JIANG Li, DONG Xingting, KANG Xiulin, LIU Lifang. Identification of chemical constituents in Fufang Shechuangzi Xiji（Lotion）by UPLC-Q-TOF-MS/MS[J]. Journal of China Pharmaceutical University, 2022, 53(4): 452-466. DOI: 10.11665/j.issn.1000-5048.20220408
[5]	XIE Jing, FAN Chunlin, XU Jie, ZHANG Jian, YE Wencai, ZHANG Xiaoqi. Alkaloids of Ervatamia pandacaqui[J]. Journal of China Pharmaceutical University, 2021, 52(3): 287-292. DOI: 10.11665/j.issn.1000-5048.20210304
[6]	ZHANG Yilun, ZHAO Xinyi, DONG Shubo, DONG Chao, SHEN Wenbin, DONG Haijuan. Isolation and identification of unknown impurities of alogliptin benzoate[J]. Journal of China Pharmaceutical University, 2020, 51(4): 488-493. DOI: 10.11665/j.issn.1000-5048.20200415
[7]	TAO Junjie, YANG Jie, WEN Xiaodong. Studies on the chemical constituents in the aerial parts of Paris polyphylla var. Yunnanensis[J]. Journal of China Pharmaceutical University, 2020, 51(4): 454-460. DOI: 10.11665/j.issn.1000-5048.20200410
[8]	HUANG Qilong, ZHANG Wanjin, LI Yan, CHEN Juan, ZHOU Baoping, ZOU Xiaohan, ZHANG Chunlei, CAO Zhengyu. Alkaloid constituents from Corydalis decumbens[J]. Journal of China Pharmaceutical University, 2017, 48(5): 563-567. DOI: 10.11665/j.issn.1000-5048.20170509
[9]	YANG Hanbing, YANG Wenqiang, SONG Min, ZHANG Jian, ZHANG Xiaoqi, YE Wencai. Phenolic constituents of Galla Chinensis and their antiviral activities[J]. Journal of China Pharmaceutical University, 2016, 47(5): 566-569. DOI: 10.11665/j.issn.1000-5048.20160510
[10]	ZHOU Yongmei, SHI Xianming, MA Lei, ZHANG Sifang. Isolation and identification of Withaphysalins from Physalis minima[J]. Journal of China Pharmaceutical University, 2015, 46(1): 62-65. DOI: 10.11665/j.issn.1000-5048.20150107

Cited By

Cited by

Periodical cited type(1)

吴素萍. 溴夫定治疗老年带状疱疹的效果与安全性分析. 中国实用医药. 2023(18): 132-135 .

Other cited types(0)

Get Citation

PDF

XML

Article views (411) PDF downloads (697) Cited by(1)

Turn off MathJax

Article Contents

Abstract

References

Protective effect and mechanism of Jinshuibao tablet on acute kidney injury induced by cisplatin in rats

Abstract

References

Related Articles

Cited by

Periodical cited type(1)

Other cited types(0)

Catalog

Protective effect and mechanism of Jinshuibao tablet on acute kidney injury induced by cisplatin in rats

Abstract

References

Related Articles

Cited by

Periodical cited type(1)

Other cited types(0)

Catalog

Export File

Citation

Format

Content