基于荧光素酶生物发光检测方法的研究及其应用进展

Advance on detection method and application based on bioluminescence of luciferase

摘要: 生物发光现象广泛存在于自然界中，不管是在陆地或是海洋都有发光生物的踪迹。其中基于荧光素酶的生物发光系统被广泛研究，启发着人类在基因、表观遗传等方面进行探索，并且开发出一系列相关的检测方法，用于体内体外各方面研究。本文从生物发光系统、荧光素酶的种类以及荧光素酶生物发光检测方法的开发与应用这几个方面进行总结，简要概括近年来基于荧光素酶生物发光检测的研究进展。

Abstract: Bioluminescence is a widespread phenomenon in nature, and luminescent organisms can be found both on land and in the ocean. Among them, luciferase based bioluminescence systems have been widely studied, inspiring the exploration of genetic and epigenetic aspects and the development of a series of related assays for in vivo and in vitro studies. This paper summarizes the recent developments of luciferase based bioluminescence assays in terms of bioluminescence systems, types of luciferases, and the development and application of luciferase bioluminescence assays.