• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
ZHAO Xun, YUAN Yaozuo, ZHANG Mei, TAN Li. Determination of pyrazinamide and its related substances by HPLC[J]. Journal of China Pharmaceutical University, 2013, 44(6): 536-539. DOI: 10.11665/j.issn.1000-5048.20130609
Citation: ZHAO Xun, YUAN Yaozuo, ZHANG Mei, TAN Li. Determination of pyrazinamide and its related substances by HPLC[J]. Journal of China Pharmaceutical University, 2013, 44(6): 536-539. DOI: 10.11665/j.issn.1000-5048.20130609
shu

Determination of pyrazinamide and its related substances by HPLC

More Information
    Graphical Abstract
    • Abstract
  • An HPLC method was established for the determination of pyrazinamide and its related substances. The method was carried out on a TSK-gel ODS-gel C18 column(250 mm×4. 6 mm, 5 μm)with a mobile phase consisting of methanol-water(pH 3. 0 adjusted with glacial acetic acid)(8 ∶92)at the flow rate of 1. 0 mL/min. The detection wavelength was set at 268 nm and the column temperature was kept at 35 °C. There was fair resolution between pyrazinamide and its related substance. The calibration curves were linear over the range of 0. 4 μg/mL and 250 μg/mL(r=1. 00). The limits of detection and quantitation were 6. 5 ng/mL and 40. 5 ng/mL, respectively. The contents data of nine batches of pyrazinamide determined by the developed method were consistent with those measured by non-aqueous potentiometric titration(BP 2012). The established HPLC method is specific, accurate, durable and easy to operate, has been proposed to be used in the Chinese Pharmacopoeia and the International Pharmacopoeia. It could be applied for the determination of pyrazinamide and its related substances.
    • FullText(HTML)
    • References (8)
  • [1]
    Zou Q,Zheng YY,Li BD.Synthesis of pyrazinamide[J].Jiangsu Chem Ind(江苏化工),2005,6(33)Suppliment:124-126.
    [2]
    Zhai J.Synthesis of 2-Cyanopyrazine[D]. Hangzhou: Zhejiang University,2003.
    [3]
    Liu JH,Cheng YB,Li XL.Determiantion of pyrazinamide tablets by RP-HPLC[J].Jiangsu Pharm Clin Res(江苏药学与临床研究),1999,7(3):17-18.
    [4]
    Pang QY,Lu Y,Yu L.Analytical separation of rifampicin,isoniazid and pyrazinamide in compound rifampicin tablets by RP-HPLC[J].J Pharm Anal(药物分析杂志),1998,18(4):259-261.
    [5]
    Li QL,Jiang XM,Shi CY.Determination of rifampicin,isoniazid and pyrazinamide in compound rifamipicin tablet by HPLC[J].Chin J Antibiot(中国抗生素杂志),2002,27(9):570-571.
    [6]
    Li LX,Ao SC,Duan XY.Determination of pyrazinamide by HPLC[J].Chin J Hosp Pharm(中国医院药学杂志),2002,5(22):317.
    [7]
    Yan XM,Ye Y.Determination of isoniazid,rifampicin and pyrazinamide in blood serum by HPLC[J].Jiangxi Med(江西医药),2008,43(11):1 231-1 233.
    [8]
    Cai ZY,Zhou BH,Li JY.Determination the content of isoniazid and pyrazinamide in tablets by ion-pair-RP-HPLC[J].Chin Pharm(中国药师),2005,8(7):562-563.
    • Related Articles

  • Related Articles

    [1]YANG Ruocong, DUAN Feipeng, CHAO Jiahong, TIAN Pengpeng, YAN Zhiyong, LI Shaojing. Advances of microRNA activity in innate immunity[J]. Journal of China Pharmaceutical University, 2017, 48(4): 396-406. DOI: 10.11665/j.issn.1000-5048.20170403
    [2]SI Lianghui, MA Hui, ZHOU Jinpei, ZHANG Huibin. Advances in antidiabetic small molecule ABHD6 inhibitors[J]. Journal of China Pharmaceutical University, 2017, 48(2): 125-134. DOI: 10.11665/j.issn.1000-5048.20170201
    [3]WU Shiwei, ZHANG Hui, LI Qianbin, HU Gaoyun. Advances in thioredoxin reductase and its inhibitors[J]. Journal of China Pharmaceutical University, 2016, 47(5): 511-520. DOI: 10.11665/j.issn.1000-5048.20160502
    [4]XIN Minhang, ZHANG Sanqi. Advances in PI3Kδ selective inhibitors[J]. Journal of China Pharmaceutical University, 2016, 47(5): 503-510. DOI: 10.11665/j.issn.1000-5048.20160501
    [5]YAO Guilin, WANG Haiyong, LU Tao. Advances of the uricosuric drugs[J]. Journal of China Pharmaceutical University, 2016, 47(4): 491-496. DOI: 10.11665/j.issn.1000-5048.20160417
    [6]LIU Kejun, ZHANG Zhimin, RAN Ting, CHEN Hongli, LU Tao, CHEN Yadong. Advances in BET bromodomain protein inhibitors[J]. Journal of China Pharmaceutical University, 2015, 46(3): 264-271. DOI: 10.11665/j.issn.1000-5048.20150302
    [7]LI Tonghui, GUO Hao, LU Tao, WANG Yue, LU Shuai, TANG Weifang. Advances in the research of FLT3 inhibitors for acute myeloid leukemia[J]. Journal of China Pharmaceutical University, 2015, 46(2): 153-161. DOI: 10.11665/j.issn.1000-5048.20150203
    [8]LI Chunhong, DU Hongjin, WEN Xiao′an, SUN Hongbin. Advances in inhibitors of MDM2 and MDM4[J]. Journal of China Pharmaceutical University, 2015, 46(1): 1-15. DOI: 10.11665/j.issn.1000-5048.20150101
    [9]Advances in the research of factor Xa inhibitors[J]. Journal of China Pharmaceutical University, 2010, 41(2): 104-111.
    [10]Advances in Selective COX-2 Inhibitors[J]. Journal of China Pharmaceutical University, 2003, (3): 1-6.

Catalog

    Get Citation
    PDF
    XML
    Article views (1080) PDF downloads (1553) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：292743

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return