Advanced Search
YUAN Wenbo, DING Yongjuan, XU Jingjing. Determination of 5-fluorouracil in serum by direct protein precipitation-ultra performance liquid chromatography[J]. Journal of China Pharmaceutical University, 2015, 46(1): 81-84. DOI: 10.11665/j.issn.1000-5048.20150111
Citation: YUAN Wenbo, DING Yongjuan, XU Jingjing. Determination of 5-fluorouracil in serum by direct protein precipitation-ultra performance liquid chromatography[J]. Journal of China Pharmaceutical University, 2015, 46(1): 81-84. DOI: 10.11665/j.issn.1000-5048.20150111
shu

Determination of 5-fluorouracil in serum by direct protein precipitation-ultra performance liquid chromatography

More Information
    Graphical Abstract
    • Abstract
  • A method based on direct protein precipitation-ultra performance liquid chromatography was established for the determination of trace 5-fluorouracil in serum. The samples were pretreated with 6% perchloric acid as the precipitant and 5-chlorouracil as the internal standard, and the supernatant separated after vortex mixing and high speed centrifugation was used as the test sample. The separation was carried out with an Acquity HSS T3 column and the mobile phase consisting of water and acetonitrile, and the flow rate was 0. 2 mL/min; the injection volume was 5 μL, and the column temperature was 20 °C. The key parameters of this method such as specificity, accuracy, precision and linearity, were validated. Compared with other methods, this method can complete the analysis with significantly reduced time and cost. The established method is useful for therapeutic drug monitoring of 5-fluorouracil plasma concentration and it has been successfully applied in clinical cases.
    • FullText(HTML)
    • References (17)
  • [1]
    Chu E.Clinical colorectal cancer:“ode to 5-fluorouracil”[J].Clin Colorectal Cancer,2007,6(9):609.
    [2]
    Shitara K,Matsuo K,Mizota A,et al.Association of fluoropyrimidines,platinum agents,taxanes,and irinotecan in any line of chemotherapy with survival in patients with advanced gastric cancer[J].Gastric Cancer,2011,14(2):155-160.
    [3]
    Leong T.Chemotherapy and radiotherapy in the management of gastric cancer[J].Curr Opin Gastroenterol,2005,21(6):673-678.
    [4]
    Douillard JY,Cunningham D,Roth AD,et al.Irinotecan combined with fluorouracil compared with fluorouracil alone as first-line treatment for metastatic colorectal cancer:a multicentre randomized trial[J].Lancet,2000,3(55):1041-1047.
    [5]
    Erick G,Remy D,Jacques J,et al.Individual fluorouracil dose adjustment based on pharmacokinetic follow-up compared with conventional dosage:results of a multicenter randomized trial of patients with metastatic colorectal cancer[J].J Clin Oncol,2008,26(28):2099-2105.
    [6]
    Peng RJ,Dong QM,Shi YX,et al.Correlative analysis between serum dihydropyrimidine dehydrogenase activity,concentration of 5-fluorouracil and adverse events in the treatment of advanced gastric cancer patients[J].Chin J Cancer(癌症),2006,25(8):1039-1043.
    [7]
    Cai X,Xue P,Song WF,et al.The role of pharmacokinetic monitoring of fluorouracil in improvement of efficacy and reduction of adverse reactions for patients with advanced gastric cancer[J].Tumor(肿瘤),2011,31(10):930-936.
    [8]
    Yu JX,Cai J,Shi LM,et al.Determination of fluorouracil concentration in tissues by HPLC[J].Chin J Drug Appl Monit(中国药物应用与监测),2011,8(1):21-23.
    [9]
    Anderson D,Blesing DJ,Blesing C,et al.Simultaneous gas chromatographic-mass spectrophotometric determination of α-fluoro-β-alanine and 5-fluorouracil in plasma[J].J Chromatogr B,1997,688(1):87-93.
    [10]
    Milano G,Roman P,Khater R,et al.Dose versus pharmacokinetics for predicting tolerance to 5-day continuous infusion of 5-FU[J].Int J Cancer,1988,41(4):537-541.
    [11]
    Zhang FH,Guzha NE,Shen W,et al.Determination of 5-fluorouracil in human plasma by HPLC[J].Gansu Med J(甘肃医药),2009,28(5):384-385.
    [12]
    Guo ZL,Fan J.Comparison study of solid-phase extraction and protein precipitation for the determination of vancomycin in human serum by HPLC[J].Chin Hosp Pharm J(中国医院药学杂志),2013,33(9):745-747.
    [13]
    Yuran C,Jing Z.Development and validation of a new UPLC method for vancomycin and comparison to FPIA(TDx)[C/OL]// The 11th National Academic Symposium of Chemotherapeutic Pharmacology.Guiyang,2012.[2014-10-9] .http://cpfd.cnki.com.cn/Article/CPFDTOTAL-ZGYS201207001014.htm
    [14]
    Li HL,Qiao J,Cao M,et al.Comparison of six different precipitants for the determination of vancomycin and norvancomycin in human plasma[J].Shanxi Med J(山西医药杂志),2009,38(12):1154-1155.
    [15]
    Jelena J,Hanna SS,Johan P,et al.Comparison of UPLC and HPLC for analysis of dietary folates[J].Chromatographia,2011,73(3/4):219-225.
    [16]
    Asta O,Vilma O,Vida V,et al.UPLC a powerful tool for the separation of imidazolium ionic liquid cations[J].Chromatographia,2011,73(3/4):17-24.
    [17]
    Quanyun AX,Reza K,Jay KT,et al.Quantitative determination of busulfan in human plasma by UPLC[J].Chromatographia,2009,70(9/10):1505-1510.
    • Related Articles

  • Related Articles

    [1]ZHOU Ping, LI Li, WU Lulu, GU Chenhao, TIAN Huimin, REN Xiaoming, ZHANG Haijun, WU Jiaquan. Determination of osimertinib mesylate by HPLC[J]. Journal of China Pharmaceutical University, 2017, 48(3): 322-327. DOI: 10.11665/j.issn.1000-5048.20170312
    [2]LIU Yongli, ZHAO Zhenxia, LI Dongmei, FENG Li. Simultaneous determination of seven components in Huoxiang Zhengqi liquid by ultra-performance liquid chromatography[J]. Journal of China Pharmaceutical University, 2013, 44(3): 249-252. DOI: 10.11665/j.issn.1000-5048.20130312
    [3]XIE Feng, LI Zhan-bin, TAN Hong, WANG Yan-jiao. Determination of chromium in capsule shells by ultrasonic wave promoting dissolution-graphite furnace atomic absorption spectrometry[J]. Journal of China Pharmaceutical University, 2012, 43(3): 281-282.
    [4]ZHANG Qiang, FENG Wen-jun, LI Xiao-dong. Robustness test on determination of luteolin-7- O-glucoside in Flos Lonicerae[J]. Journal of China Pharmaceutical University, 2010, 41(6): 555-557.
    [5]Determination of methacycline and its related substances in the bulk material of methacycline hydrochloride and its preparations by HPLC[J]. Journal of China Pharmaceutical University, 2010, 41(3): 268-272.
    [6]ZHANG Jin-lin, YUAN Yao-zuo, QIAN Wen, ZHANG Mei. Determination of secnidazole and its related substances by HPLC[J]. Journal of China Pharmaceutical University, 2009, 40(6): 527-530.
    [7]Determination of Ephedrine Hydrochloride by Chelating with Cu2+[J]. Journal of China Pharmaceutical University, 2000, (4): 40-42.
    [8]Determination of Dextromethorphan Hydrobromide Contained in Yumei Granules by Acid Dye Colorimetry[J]. Journal of China Pharmaceutical University, 1998, (6): 437-439.
    [9]Pan Yang, Ma Guoxiang, Guo Liwei, zhang Yaoqi, wu Jun. Determination of Chlorogenic Acid in Zijin-Jieduyin by Reversed-Phase HPLC[J]. Journal of China Pharmaceutical University, 1995, (4).
    [10]SYNTHESIS OF ACYCLONUCLEOSIDE DERIVATIVES AND ANALOGUES OF 5-FLUOROURACIL[J]. Journal of China Pharmaceutical University, 1986, (3): 161-166.

  • Cited by

    Periodical cited type(2)

    1. 冯惠，王晓辉，王菊，冯长君. 培氟沙星均三唑硫醚类化合物抗增殖活性的3D-QSAR研究与分子设计. 云南大学学报(自然科学版). 2021(01): 142-146 .
    2. 唐自强，刘长宁，冯长君. 培氟沙星均三唑硫醚衍生物抗肝癌活性的CoMFA模型与分子设计. 化学通报. 2020(10): 935-939 .

    Other cited types(0)

Catalog

    Get Citation
    PDF
    XML
    Article views (1160) PDF downloads (1928) Cited by(2)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：436282

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return