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SHEN Yanli, JI Xiaonan, GAO Xiangdong. Combination of scFv-AFP with doxorubicin inhibits cell proliferation in hepatocellular carcinoma cell Huh7[J]. Journal of China Pharmaceutical University, 2016, 47(1): 101-105. DOI: 10.11665/j.issn.1000-5048.20160115
Citation: SHEN Yanli, JI Xiaonan, GAO Xiangdong. Combination of scFv-AFP with doxorubicin inhibits cell proliferation in hepatocellular carcinoma cell Huh7[J]. Journal of China Pharmaceutical University, 2016, 47(1): 101-105. DOI: 10.11665/j.issn.1000-5048.20160115
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Combination of scFv-AFP with doxorubicin inhibits cell proliferation in hepatocellular carcinoma cell Huh7

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  • This study was to investigate the inhibitory effects of single-chain variable fragment of alpha fetoprotein(scFv-AFP)in combination with doxorubicin on the proliferation of human hepatocellular carcinoma cell lines Huh7. Huh7 cells were treated with different concentrations of scFv-AFP or doxorubcin alone or their combination. The inhibitory effects were detected by MTT assay, and cycle arrest and apoptosis of Huh7 cells were analyzed by flow cytometry in different groups using PI and Annexin V/PI-staining, respectively. Results showed that scFv-AFP, doxorubicin alone or in combinations dose-dependently inhibited the proliferation of Huh7, and a synergistic effect was observed in their combined action. The combined therapy resulted in significantly higher apoptosis than those in other groups(P< 0. 05). scFv-AFP(40 μg/mL)markedly blocked the Huh7 cell progression by arresting the cells in the G0/G1 phase, and the percentage of cells in S phase decreased dramatically(P< 0. 05); and scFv-AFP combined with doxorubicin blocked the Huh7 cell progression by arresting the cells in G2/M phase(P< 0. 01).
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  • [1]
    Zhao Y,Wang Q,Deng X,et al.Quantitative assessment of the association between GSTP1 gene Ile105Val polymorphism and susceptibility to hepatocellular carcinoma[J].Tumour Biol,2013,34(4):2121-2126.
    [2]
    Zhu MY,Xia H,Li MS,et al.Alpha fetoprotein can induce malignant transformation of liver cells and be used as a therapeutic target for hepatocellular carcinoma[J].World Chin J Dige(世界华人消化杂志),2014, 22(8):1070-1075.
    [3]
    Mc Govern,B J.Epidemiology and natural history of hepatitis B[J].Semin Liver Dis,2005, 25(suppl):3-8.
    [4]
    Gupta R,Vyas P,Enver T.Molecular targeting of cancer stem cells[J].Cell Stem Cell,2009, 5(2):125-126.
    [5]
    Liu JT,Jiao YY,Zhang YH.Effect of 1B50-1,a novel antibody,combined with chemotherapeutic drugs on proliferation of hepatocellular carcinoma cells[J].Chin J New Drugs(中国新药杂志),2015, 24(2):157-161.
    [6]
    Lu HL,Ji XN,Feng LY,et al.Optimization of expression conditions of scFv-AFP and its immunologic activity[J].J China Pharma Univ(中国药科大学学报),2012,43(5):460-465.
    [7]
    Li M,Zhu M,Li W,et al.Alpha-fetoprotein receptor as an early indicator of HBx-driven hepatocarcinogenesis and its applications in tracing cancer cell metastasis[J].Cancer Lett,2013, 330(2):170-180.
    [8]
    Hung TM,Hu RH,Ho CM,et al.Downregulation of alpha fetoprotein expression by LHX4:a critical role in hepatocarcinogenesis[J].Carcinogenesis,2011, 32(12):1815-1823.
    [9]
    Zheng L,Gong W,Liang P,et al.Effects of AFP-activated PI3K/Akt signaling pathway on cell proliferation of liver cancer[J].Tumour Biol,2014, 35(5):4095-4099.
    [10]
    Li M,Li H,Li C,et al.Alpha fetoprotein is a novel protein-binding partner for caspase-3 and blocks the apoptotic signaling pathway in human hepatoma cells[J].Int J Cancer,2009, 124(12):2845-2854.
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