Expression and purification of human placental growth factor 2 using the<i> Pichia pastoris</i> expression system

LIANG Xiaoqing; YAN Pengfei; LIU Yu

doi:10.11665/j.issn.1000-5048.20180318

Journal of China Pharmaceutical University > 2018 > 49(3): 376-380. > DOI: 10.11665/j.issn.1000-5048.20180318

LIANG Xiaoqing, YAN Pengfei, LIU Yu. Expression and purification of human placental growth factor 2 using the Pichia pastoris expression system[J]. Journal of China Pharmaceutical University, 2018, 49(3): 376-380. DOI: 10.11665/j.issn.1000-5048.20180318

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Expression and purification of human placental growth factor 2 using the Pichia pastoris expression system

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To prepare the human placental growth factor 2(PIGF-2), human PIGF-2 gene was cloned into pPIC9K vector to construct the recombinant pPIC9K-PIGF-2 vector. Linearized recombinant pPIC9K-PIGF-2 was transformed into Pichia pastoris by electroporation. YPD-Geneticin plate was used to screen geneticin hyper-resistant colonies. The positive colonies were verified by PCR. Results of SDS-PAGE and Western blot showed that recombinant human PIGF-2 was expressed after being induced by methanol. Using its characteristic heparin binding, recombinant human PIGF-2 was successfully purified by heparin affinity column chromatography.
- placental growth factor 2(PIGF-2),
- Pichia pastoris,
- purify

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Expression and purification of human placental growth factor 2 using the Pichia pastoris expression system

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