Applications and progress of chemical derivatization in targeted metabolomics LC-MS analysis

ZHANG Xien; WANG Di; XU Fengguo

doi:10.11665/j.issn.1000-5048.20210104

Journal of China Pharmaceutical University > 2021 > 52(1): 31-37. > DOI: 10.11665/j.issn.1000-5048.20210104

ZHANG Xien, WANG Di, XU Fengguo. Applications and progress of chemical derivatization in targeted metabolomics LC-MS analysis[J]. Journal of China Pharmaceutical University, 2021, 52(1): 31-37. DOI: 10.11665/j.issn.1000-5048.20210104

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Applications and progress of chemical derivatization in targeted metabolomics LC-MS analysis

Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing 210009, China

Funds: This study was supported by the National Natural Science Foundation of China (No.82073812, No.81773682, No.81773861) and the Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province(No.BK20180027)

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Received Date: July 25, 2020
Revised Date: September 21, 2020

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Abstract

Abstract

The changes of metabolic profile are closely related to external stimulus, and the concentration of the metabolite can directly reflect the physiological or pathological states of organisms. Therefore, the quantitative detection of metabolites is necessary. However, traditional targeted metabolomic methods have such drawbacks as narrow coverage and low sensitivity. In recent years, derivatization techniques have developed rapidly in the field of metabolomics. Derivatization reagents for amine, hydroxyl, carboxyl, carbonyl, hydrosulphonyl and other groups have been used in metabolomics research. This paper introduces various derivatization reactions and their applications according to group classification and reviewes the characteristics of multi-group derivatization techniques, with a propect of their research directions and challenges.
- derivatization,
- LC-MS,
- metabolomics,
- quantitative analysis

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References

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