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HE Mingzhe, PENG Ying, WANG Guangji, A Jiye, ZHENG Yiwen, SUN Jianguo. Mechanism of sodium salicylate-induced damage to HEI-OC1 cells based on metabonomics[J]. Journal of China Pharmaceutical University, 2021, 52(5): 566-572. DOI: 10.11665/j.issn.1000-5048.20210508
Citation: HE Mingzhe, PENG Ying, WANG Guangji, A Jiye, ZHENG Yiwen, SUN Jianguo. Mechanism of sodium salicylate-induced damage to HEI-OC1 cells based on metabonomics[J]. Journal of China Pharmaceutical University, 2021, 52(5): 566-572. DOI: 10.11665/j.issn.1000-5048.20210508
shu

Mechanism of sodium salicylate-induced damage to HEI-OC1 cells based on metabonomics

  • 1.

    Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China

  • 2.

    Department of Pharmacology and Toxicology, School of Biomedical Sciences, University of Otago, Dunedin 9054, New Zealand

Funds: This study was supported by the National Key Research and Development Program for Intergovernmental Cooperation in International Science and Technology Innovation (No.2017YFE0109600)
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  • Received Date: April 06, 2021
  • Revised Date: September 13, 2021
    Graphical Abstract
    • Abstract
  • The effect of sodium salicylate on the endogenous metabolism of hair cell-like cells (HEI-OC1).of mice was analyzed based on liquid chromatography-quadrupole time of flight mass spectrometry (LC-Q-TOF/MS).Firstly, HEI-OC1 cells were treated with different concentrations of sodium salicylate, and cell survival was examined by the CCK-8 method. Next, sodium salicylate was administered for different duration to observe the changes in cell morphology. Inter-group differential metabolites were screened out, and the associated metabolic pathways were analyzed based on metabonomic technology.Results showed that sodium salicylate could significantly inhibit the survival rate of HEI-OC1 cells, and that, as the concentration increased, the inhibitory effect became stronger. Also, the cell morphology could be elongated after administration and return to normal after withdrawal.Eighteen differential metabolites such as orotic acid, uridine and aspartic acid were screened out after treatment of sodium salicylate, which mainly involving two possible metabolic pathways, namely the metabolism of alanine, aspartic acid and glutamic acid, and that of pyrimidine.In summary, the application of metabolomics technology to evaluate the effect of sodium salicylate on hair cells from the microscopic perspective can provide new ideas for the study of sodium salicylate ototoxicity and development of tinnitus.
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    • References (18)
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